In this study, we screened the expression stability of six reference genes (18S rRNA, β-actin, GAPDH, EF1a, B2M, and HPRT1) in hybrid yellow catfish (n = 6), considering SBM levels, sampling time points, different tissues. Four statistical programs, BestKeeper, NormFinder, Genorm, Delta Ct, combined with a method that comprehensively considered all results, were used to evaluate these systemati...

In this work, a commercially available autosampler was adapted to perform direct liquid microjunction (LMJ) surface sampling followed by a high-pressure liquid chromatography (HPLC) separation of the extract components and detection with electrospray ionization mass spectrometry (ESI-MS). To illustrate the utility of coupling a separation with this direct liquid extraction based surface samplin...

extracelllar matrix (ecm) materials from mammalian tissues have been broadly used for tissue engineering for animal models. while decellularization of tissues such as bladder, heart valve, knee meniscus and tendon has been performed, there are a few reports for articular cartilage. in this study we aimed to develop a technique to decellularize bovine articular cartilage as a biological construc...

Different species of Streptomyces are common in most types of soil. Some certain species are plant pathogenic especially on potato. This study was conducted to evaluate genetic diversity among some local Streptomyces spp. strains isolated from soil and infected tubers in potato farms in northwest of Iran. Repetitive DNA elements (BOX, ERIC and REP) as genetic markers were used for diversity stu...

background: trace elements play an important role in a number of biological processes. astaxanthin (asx), a carotoid pigment found in certain marine plant and animals, has shown anti cancer and anti free radical properties. this work intended to understand the effect of astaxanthin in breast cancer (invasive ductal carcinoma, idc) by using micro-pixe method. for this aim the concentration of tr...

The aim of this study was to investigate the therapeutic effects of propolis for the toxicity of cypermethrin (CYP) on histopathological changes in tissues of rainbow trout (Oncorhynchus mykiss). CYP is one of the most toxic pyrethroids highly for the aquatic organisms. The fish were exposed to three sublethal concentrations of CYP (0.0041, 0.0082 and 0.0123 ppm). In addition, different concent...

background: nowadays use of specific antivenin for latrodectism is considered as the most effective treatment in the world. this study was undertaken to investigate the efficacy of specific antivenom against histopathological complications caused by latrodectus dahli venom on liver, heart and kidneys tissues within 72 h. methods: two groups were selected, each one contained 6 male new zealand r...

Aim: Three species of fish, Notoptrus notoptrus, Channa punctatus and Glossogobius giuris, were shown to accumulate heavy metals like Zn,Fe,Ni,Pb,Cd, Cu in their gut-free bodies, liver, gills. Methodology: These fish inhabit the Kapila River Nanjangud area Mysore district. Result: Fish tissues (muscle, gills liver) sediments found contain Fe, Pb, Ni, Zn pollution. It was also determined that th...