OBJECTIVE To investigate the possible stimulating mechanism of Huoxue Bushen Mixture (HXBSM), a traditional Chinese compound medicine, on hair growth of mice via measuring the variance of skin blood vessel neogenesis and vascular endothelial growth factor (VEGF) expression in the hair follicle. METHODS Hot rosin and paraffin mixture depilation were used to induce C57BL/6 mice hair follicle to...

Although earlier studies focused on the hormonal regulation of antral and preovulatory follicles, recent studies indicate the importance of the hormonal control mechanism for preantral follicles. The endocrine hormone FSH is not only a survival factor for early antral follicles but also a potent growth and differentiation factor for preantral follicles. In addition, KGF secreted by theca cells ...

The extracellular matrix (ECM) provides a three-dimensional structure that promotes and regulates cell adhesion and provides signals that direct the cellular processes leading to tissue development. In this report, synthetic matrices that present defined ECM components were employed to investigate these signaling effects on tissue formation using ovarian follicle maturation as a model system. I...

the aim of the present study was to investigate the effect of follicle stimulating hormone (fsh) on survival, activation and growth of ovine primordial follicles using histological studies. pieces of ovine ovarian cortex were obtained and cultured for 6 days in minimum essential medium supplemented with or without fsh (50 ng/ml). these fragments were then process to be used for histology compar...

Follicle deviation during bovine follicular waves is characterized by continued growth of a developing dominant follicle and reduction or cessation of growth of subordinate follicles. Characteristics of follicle deviation for waves with a single dominant follicle were compared between wave 1 (begins near ovulation; n = 15) and wave 2 (n = 15). Follicles were defined as F1 (largest), F2, and F3,...

Previous studies relating to the effect on whisker growth of removal of various components of the whisker follicle have shown that both after removal of the dermal papilla alone and after removal of as much as the lower third of the follicle, regeneration of new dermal papillae and then the generation of whiskers may occur (Oliver, 1966 a, b). Histological studies of this regenerative phenomeno...

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

Background: Setting up an in vitro follicle culture system that resembles in vivo ovary condition has high value in research. Additionally, expression evaluation of folliculogenesis involved genes could lead us to the designing of better culture system. Materials and Methods: ovaries of 12-day-old female NMRI mice were removed, 100-130 μm pre-antral follicles were mechanically isolated from fre...

Studies in cattle assessing changes in number and size of antral follicles, concentrations of estradiol, androgens and progesterone in serum and follicular fluid, and numbers of gonadotropin receptors per follicle during repetitive estrous cycles and postpartum anestrus are reviewed. The rate of growth of small follicles (1 to 3 mm) into larger follicles increases as the estrous cycle progresse...

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...