background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

Carbon nanodots (CDs) with superior fluorescence performance were obtained by hydrothermal method using tomatoes as raw materials. When the ultraviolet absorption band of tartrazine and spectrum CDs have complementary overlaps, fluorescent internal filter effect (IFE) occurred. Furthermore, degree quenching intensity has an excellent linear correlation concentration. And based on this principle...

Polymeric fluorescent nanoparticles with covalently embedded perylene fluorophores were developed by facile synthesis strategy and their advanced features of extremely high fluorescence intensity, non-photoblinking and excellent photostability were experimentally confirmed at the single nanoparticle level.

Fading is one of the major obstacles to reliable observation in fluorescence microscopy. Using a confocal laser scanning microscope (CLSM) coupled to a computer, we quantitatively measured fading of fluorescence to formulate an equation, evaluated the anti-fading ability of several anti-fading media, and restored the faded images to the original level according to this equation. NIH 3T3 cells w...

We investigate the two-photon fluorescence (TPF) of conjugated polymer (CP) microspheres with diameters up to tens micrometers. Two polymers, emitting in either violet or red, were first synthesized and characterized terms their one-photon three-dimensional internal microstructure. Under femtosecond infrared excitation, both types showed a strong TPF, which was investigated by excitation intens...

A fluorescence-based method using the cell sorter has been devised to separate rat lung fibroblasts into subpopulations. Type I or type III collagen antiserum was used as the primary antibody to react with parent rat lung fibroblasts. This was followed by a fluorescein-conjugated secondary antibody. Specificity of the primary collagen antibody was determined using a monoclonal beta-actin antibo...