Different individuals possess slightly different genetic information and show genetically-determined differences in several enzyme activities due to genetic variability. Following an integrated approach, we studied the polymorphisms and methylation of sites contained in the 5' flanking region of the metabolizing enzyme CYP2E1 in correlation to its expression in both tumor and non-neoplastic liv...

The nucleotide sequence of the Adh region of Drosophila mojavensis has been completed and the region found to contain a pseudogene, Adh-2 and Adh-1 arranged in that order. Comparison of the sequence divergence of these genes to one another and to the Adh region of Drosophila mulleri and other species has allowed the development of a model for the evolution of the duplication of the Adh genes. T...

Chromatin in the 5' flanking region of the transcriptionally active chicken adult beta(beta A) globin gene is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts ...

Platelet-derived growth factor (PDGF) is a heterodimeric glycoprotein consisting of A and B chains. A functional promoter had been identified in the 5' flanking region of the human PDGF-A chain gene. We found that the PDGF-A chain promoter region possesses the potential for bidirectional activity. This bidirectional promoter activity is influenced by the 5'-untranslated region (5'-UTR) and seru...

csn1s1is one of the major genes encoding milk proteins of mammals. in this study we determined allele frequencies of csn1s1-5` flanking region as well as exon 17 variants and their effects on milk traits in three indigenous cattle breeds mazandarani, golpaygani (bos indicus) and sarabi (bos taurus) and holstein cattle in iran. csn1s1*b variant was nearly fixed in holstein but ranged from 0.40 t...

The rat apolipoprotein E gene was isolated from a genomic library by screening with a cDNA probe. The nucleotide sequence of the gene plus a 5' flanking region of 623 nucleotides and a 3' flanking region of 234 nucleotides was determined. The gene contained three introns, which were located in the 5' nontranslated region and in the regions coding for the signal peptide and the mature protein. A...

The human CD34 gene is expressed on early progenitor and stem cells in the bone marrow. Here we report the isolation of the human CD34 locus from a human P1 artificial chromosome (PAC) library and the characterization and evaluation of this genomic fragment for expression of reporter genes in stable cell lines and transgenic mice. We show that a 160-kb fragment spanning 110 kb of the 5' flankin...

Complete, active, immunoglobulin variable (V) 1 genes are generated by somatic rearrangement of DNA sequences during the differentiation of lymphocyte precursor cells (1, 2). In the mouse, the conventionally defined V region of the light chains, both and ~¢ types, consists of two DNA segments: VL and Je, which are separate in the germline genome. The two DNA segments are joined at the 3' end of...

The expression of keratin 18 (K18) is restricted in humans primarily to a variety of single layered or simple epithelia. However, direct introduction of a cloned K18 gene into cultured, somatic cells by DNA transfection has been shown to result in the promiscuous expression of K18 even while the endogenous mouse form of K18 (Endo B) remains silent. To determine if the cloned K18 genomic DNA fra...