نتایج جستجو برای: field gel electrophoresis

تعداد نتایج: 895744  

Journal: :Physical review letters 2007
Peter Palffy-Muhoray

In the theoretical section of their Letter, the authors claim the result that ‘‘the magnetic current density is minus one half the total current density’’ [1]. In this Comment, I argue that their argument is flawed and their conclusion is invalid. The authors consider an incident plane wave of angular frequency ! that propagates in the z-direction with its electric field linearly polarized alon...

2012
Laura García-Descalzo Eva García-López Alberto Alcázar Fernando Baquero Cristina Cid Ramon y Cajal

Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field. These techniques have become a main tool in biochemistry, molecular...

Journal: :Clinical chemistry 1999
P H Griffioen L Zwang R H van Schaik H Engel J Lindemans C M Cobbaert

BACKGROUND Increased lipoprotein(a) is a risk factor for atherosclerosis, and its concentration in serum is inversely correlated with the size of the apoliprotein(a) [apo(a)] component. The size of the apo(a) gene is determined mainly by the Kringle IV size polymorphism. We have optimized and characterized pulsed field gel electrophoresis (PFGE) for apo(a) genotyping. METHODS Established PFGE...

Journal: :Methods in molecular biology 2015
Rosa Alduina Annalisa Pisciotta

Pulsed field gel electrophoresis (PFGE) is a quick and reliable procedure to resolve DNA molecules larger than 30 kb by applying an electric field that periodically changes direction. This technique can be used to estimate genome size of a microorganism, to reveal if a genome is circular or linear, to indicate the presence of megaplasmids, and to show if a strain contains only one or more chrom...

Journal: :Journal of clinical microbiology 2000
D Turabelidze M Kotetishvili A Kreger J G Morris A Sulakvelidze

A rapid protocol for subtyping vancomycin-resistant enterococci by pulsed-field gel electrophoresis is reported. The procedure is simple and potentially cost-effective and allows reproducible subtyping of the strains in approximately 1 day.

Journal: :Genetics and molecular research : GMR 2013
M P S Barros V M Silveira-Filho R H F B Lins M B M Oliveira A M P Almeida T C Leal-Balbino

We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak strains, as identical pulsotype patterns ...

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