To enhance the antithrombotic properties of recombinant glycoprotein VI fragment crystallizable (GPVI-Fc), the authors incubated GPVI-Fc with anti-human Fc antibodies to cross-link the Fc tails of GPVI-Fc. Cross-linking potentiated the inhibition of human plaque- and collagen-induced platelet aggregation by GPVI-Fc under static and flow conditions without increasing bleeding time in vitro. Cros...

Peripheral blood lymphocytes from 10 nonallergic donors and 7 patients suffering from seasonal allergic rhinitis and receiving desensitization therapy were analyzed by rosette assays for Fc receptors for IgE (Fc epsilon R) and IgG (Fc gamma R) before, during and after the grass pollen season. Six of seven patients had moderately elevated IgE levels (330 +/- 268 IU/ml), all had high titers of sk...

Interest in manipulating the immunosuppressive powers of Foxp3-expressing T regulatory cells as an immunotherapy has been tempered by their reported ability to produce proinflammatory cytokines when manipulated in vitro, or in vivo. Understanding processes that can limit this potentially deleterious effect of Treg cells in a therapeutic setting is therefore important. Here, we have studied this...

This article explores the flipped-classroom learning cycle and traditional blended-learning from experiential learning-theory perspective, according to which we proposed three teaching strategies: high-flipped classroom (high-FC), low-flipped (low-FC), Traditional Blended Learning (traditional BL). And, strategies are adopted in course of Enterprise Resource Planning System. The purpose is inve...

Aberrant activation of Wnt signaling plays an important role in hepatocarcinogenesis. In addition to direct effects on tumor cells, Wnt signaling might be involved in the organization of tumor microenvironment. In this study, we have explored whether Wnt signaling blockade by exogenous expression of Wnt antagonists could inhibit tumor angiogenesis and control tumor growth. Human Wnt inhibitory ...

Dendritic cells (DCs) capture immune complexes (ICs) via Fc receptors for immunoglobulin G (Fc R)II and elicit antigen presentation and protective antitumoral immune response in mice. Two protocols are commonly used to differentiate human monocyte-derived DCs in vitro. They associate granulocyte macrophage-colony stimulating factor with interleukin (IL)-4 or IL-13. In this study, we first asses...

The redox properties of [HIPTN(3)N]Mo complexes (where HIPTN(3)N = (3,5-(2,4,6-i-Pr(3)C(6)H(2))(2)C(6)H(3)NCH(2)CH(2))(3)N) involved in the catalytic dinitrogen reduction cycle were studied using cyclic voltammetry in fluorobenzene with Bu(4)NPF(6) as the electrolyte. MoN(2) (Mo = [HIPTN(3)N]Mo, E(1/2) = -1.96 V vs. Fc(+)/Fc at a Pt electrode), Mo≡N (E(1/2) = -2.68 V vs. Fc(+)/Fc (Pt)), and [Mo...

BACKGROUND A recently reported cocaine hydrolase (CocH3) fused with fragment crystallizable (Fc) region of human immunoglobulin G1, denoted as CocH3-Fc, is known as a promising therapeutic candidate for the treatment of cocaine overdose and addiction. A challenge for practical therapeutic use of this enzyme exists in the large-scale protein production and, therefore, it is interesting to identi...

BACKGROUND Calprotectin is a cytosolic component of neutrophils. Fecal calprotectin (FC) level is a useful marker for exacerbation of inflammatory bowel disease in children. FC may be a useful marker for necrotizing enterocolitis (NEC). OBJECTIVE To determine normal baseline levels of FC and observe dynamic changes of FC levels over the first postnatal month in very low birth weight (VLBW) in...