The purpose of this study was to investigate optimal concentrations of zoledronic acid (ZA) in terms of their effect on the proliferation, differentiation, and mineralization of primary osteoblasts (OBs) and fibroblasts (FBs). Primary OBs and FBs isolated from patients with clinical osteogenesis imperfecta (OI) and developmental dysplasia of the hip (DDH) were treated in vitro with serial conce...

Myrmecophytic Acacia species produce food bodies (FBs) to nourish ants of the Pseudomyrmex ferrugineus group, with which they live in an obligate mutualism. We investigated how the FBs are protected from exploiting nonmutualists. Two-dimensional gel electrophoresis of the FB proteomes and consecutive protein sequencing indicated the presence of several Kunitz-type protease inhibitors (PIs). PIs...

This study was conducted to investigate the effects of brown seaweed (Undaria pinnatifida) by-product and seaweed fusiforme (Hizikia fusiformis) by-product supplementation on growth performance and blood profiles including serum immunoglobulin (Ig) in broilers. Fermentation of seaweeds was conducted by Bacillus subtilis and Aspergillus oryzae. In a 5-wk feeding trial, 750 one-d-old broiler chic...

Two channel lter banks (FBs) were developed for subbandspeech coding [16]. Since then FB theory has grown to includeFBs with more than two channels, tree-structured FBs etc. Arich theory for the design and implementation of FBs exists.Wavelets were originally developed as an alternative to theSTFT for non-stationary signal analysis [26]. Since then it hasbeen realized th...

Bovine viral diarrhea virus (BVDV) isolation and antibody survey were performed using 2,758 fetal bovine sera (FBS) collected from slaughterhouses in New Zealand, Australia and the Dominican Republic, and then sent to Japan to manufacture commercial serum for cell culture use. FBS in the Dominican Republic were pooled for each several individuals, and those collected in other countries were sep...

wounds is unknown, but with more than 5 million estimated lacerations repaired in EDs in 2007, wound FBs surely occur in such a significant number that all emergency physicians (EPs) will encounter them. Wound FBs are frequently missed on initial evaluation. Seventy-five of 200 FBs encountered in a hand clinic were missed by the initial physician. Furthermore, missed FBs pose high medicolegal r...

AIM The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitr...

A cell line designated as HFB-ES was established from blastula stage embryos of H. fossilis (Singhi). The embryonic cells were harvested and maintained in Leibovitz's medium supplemented with 15% fetal bovine serum. The cell line had been subcultured for more than 90 passages in a period of 24 months. HFB-ES cells were able to grow at temperatures between 25 and 35°C with an optimum temperature...

The objectives of this study were (i) to examine the effects on rumen and blood variables when grass silage (GS) was replaced with whole crop fodder beet silage (FBS) in the diet of beef cattle, and (ii) an abrupt change from GS to FBS. Six rumen-fistulated Friesian steers (initial bodyweight (BW) 416 kg, SD 43) were offered GS ad libitum for four weeks. The dry matter (DM) consumed daily by ea...