Received for publication 26 October 1976 and in revised form 24 January 1977. INTRODUCTION The fat cell adenylate cyclase is a plasma membrane-bound enzyme which is supposed to play a central role in the stimulation of lipolysis by hormones (1). The rat fat cell enzyme reacts to a variety of hormones including catecholamines and peptide hormones such as ACTH, glucagon, and secretin (2-4). By co...

BACKGROUND/AIMS In fat cells of obese humans, basal lipolysis is increased but catecholamine-stimulated lipolysis is blunted. This is linked to decreased expression of hormone-sensitive lipase (HSL). Upon stimulation by cAMP, HSL is phosphorylated at several serine residues (P-Ser(552), P-Ser(649) and P-Ser(650)) leading to enzymatic activation. In contrast, P-Ser(554) prevents phosphorylation ...

Adipose tissue is a major component of normal rabbit marrow. Morphological considerations suggest an active role for this tissue in hematopoiesis. This hypothesis was tested by injecting 50 tCi of palmitate-1-14C intravenously into fed, hematologically normal New Zealand rabbits. The animals were sacrificed 24 hr later and the femoral marrow removed. Samples of subcutaneous and perinephric fat ...

The hydrolysis of triglyceride (TG) stored in the lipid droplets of the insect fat body is under hormonal regulation by the adipokinetic hormone (AKH), which triggers a rapid activation cAMP-dependent kinase cascade (protein kinase A (PKA)). The role of phosphorylation on two components of the lipolytic process, the TG-lipase and the lipid droplet, was investigated in fat body adipocytes. The a...

The in vitro lipolytic response to norepinephrine by rat adipocytes from epididymal, subcutaneous, perirenal, mesenteric, and omental tissue sites was studied in control and hypotensive animals. Lipolysis per millimole of triglyceride was found to be three to four times higher in mesenteric and omental fat cells than in adipocytes of the other sites sampled. The high lipolytic activity of mesen...

The role of mild kidney dysfunction in altering lipid metabolism and promoting inflammation was investigated in uninephrectomized rats (UniNX) compared to Sham-operated controls rats. The impact of UniNX was studied 1, 2, and 4 weeks after UniNX under mild food restriction at 90% of ad libitum intake to ensure the same caloric intake in both groups. UniNX resulted in the reduction of fat pad we...

Green tea, particularly epigallocatechin-3-gallate (EGCG), may affect body weight and composition, possibly by enhancing fat oxidation. The aim of this double-blind, randomized placebo-controlled cross-over study was to investigate whether 3-day supplementation with EGCG (282 mg/day) stimulates fat oxidation and lipolysis in 24 overweight subjects (age = 30 ± 2 yrs, BMI = 27.7 ± 0.3 kg/m(2)). E...

Adipose tissue is a major component of normal rabbit marrow. Morphological considerations suggest an active role for this tissue in hematopoiesis. This hypothesis was tested by injecting 50 micronCi of palmitate-1-14C intravenously into fed, hematologically normal New Zealand rabbits. The animals were sacrificed 24 hr later and the femoral marrow removed. Samples of subcutaneous and perinephric...

AIM To assess in rodent and human adipocytes the antilipolytic capacity of hexaquis(benzylammonium) decavanadate (B6V10), previously shown to exert antidiabetic effects in rodent models, such as lowering free fatty acids (FFA) and glucose circulating levels. METHODS Adipose tissue (AT) samples were obtained after informed consent from overweight women undergoing plastic surgery. Comparison of...