The present investigation deals with the purification and characterization of enzyme α-amylase from a mutant strain of Bacillus licheniformis EMS-6. A laboratory scale stirred fermentor of 7.5 L capacity was used for the enzyme production under optimal conditions. The enzyme was purified up to homogeneity level by Ammonium sulphate and ion-exchange chromatography using a fast protein liquid chr...

background diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human. objectives this study was done to assess whether pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil. materials and methods serial dilutions of pure and commercial diazinon in minimal salt medium were made ...

Little was known about biological activities of compounds from the medicinal mushroom of the genus Pholiota. A lectin from the Pholiota adiposa has now been isolated and its properties tested. The isolation procedure included ion exchange chromatography on DEAE-cellulose and CM-cellulose, and fast protein liquid chromatography-gel filtration (FPLC) on Superdex 75. The lectin was composed of two...

in this study, for the analysis of human urine samples, a novel method explained for the determination of celecoxib, a nonsteroidal anti-inflammatory drug (nsaid), using molecularly imprinted solid-phase extraction (mispe) coupled with high-performance liquid chromatography (hplc). the synthesis of the mip was performed by precipitation polymerization in methacrylic acid (maa), ethylene glycol ...

a solid phase extraction sorbent based on polyaniline/graphene nanocomposite is presented. the structure and morphology of synthesized nanocomposite were investigated by fourier transform infrared spectroscopy, field emission scanning electron microscopy, x-ray diffraction and thermal gravimetric analysis. the dispersive solid phase extraction was employed to the isolation and preconcentration ...

objective(s):the present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum. materials and methods:the hplc method was carried out by using a c18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the flow...