Following the initial report of the use of SYBR Green I for real-time polymerase chain reaction (PCR) in 1997, little attention has been given to the development of alternative intercalating dyes for this application. This is surprising considering the reported limitations of SYBR Green I, which include limited dye stability, dye-dependent PCR inhibition, and selective detection of amplicons du...

Various methods for the recovery and detection of HAV have been suggested, and molecular tests have recently provided an effective replacement for the traditional methods. Real-time RT-PCR technology offers many advantages over conventional RT-PCR in terms of rapidity and specificity. Most procedures are based on the TaqMan chemistry, but some researchers have used the SYBR Green I approach, wh...

(3Z)-Hexenol, which is commonly called leaf al­ cohol, and (2£)-hexenal, also called leaf aldehyde, are widely distributed in fresh leaves, vegetables and fruits. They are mainly responsible for the socalled “green odour” characteristic of leaves, along with other C6-compounds such as (3£")-, and (2£)-hexenols, their corresponding aldehydes (3Z)-, (3£")-hexenals, «-hexanol and «-hexanal. Thus, ...

Microalgal storage lipids are considered to be a promising source for next-generation biofuel feedstock. However, microalgal biodiesel is not yet economically feasible due to the high cost of production. One of the reasons for this is that the use of a low-cost open pond system is currently limited because of the unavoidable contamination with undesirable organisms. Extremophiles have an advant...

Green tea ('Sencha'), made from the leaves of Camellia sinensis, is the most well-researched antioxidant beverage. The major source of its antioxidant activity is polyphenols, consisting mainly of catechins (flavan-3-ols). However, little is known about the physiological effects of green tea aroma, which lacks catechins. In the present study, we performed inhalation experiments with green tea a...

Application of green fluorescent protein (GFP) as a molecular biosensor or reporter is limited by several factors especially its poor fluorescence at low pH conditions. There are conflicting data on this limitation, however, systematic studies to assess the importance of this factor for growing bacterial cultures are lacking. In this study, effect of low pH on GFP green and red fluorescence int...

Life-cycle assessment was used to evaluate the widespread installation of green roofs in a typical urban mixed-use neighborhood. Market prices of materials, construction, energy conservation, storm-water management, and greenhouse gas GHG emission reductions were used to evaluate private and social costs and benefits. Results suggest green roofs are currently not cost effective on a private cos...