A panel of 13 monoclonal antibodies to avian lipoprotein lipase (LPL) was screened for inhibition of LPL binding to primary avian adipocytes. One monoclonal antibody, designated xCAL (monoclonal antibody to chicken adipose lipoprotein lipase) 3-6a, was found to inhibit the binding of LPL to primary avian adipocytes. In solid phase assays, xCAL 3-6a inhibited the binding of LPL to both heparan s...

The regulation of lipoprotein lipase activity was studied in rat adipocytes. Incubation of fat cells for 60 mm in buffer without glucose and insulin resulted in a 50% decrease in lipoprotein lipase activity. This decrease was prevented by glucose and insulin. Inhibition of fat cell protein synthesis by cycloheximide abolished the effect of glucose and insulin and caused a rapid decay of lipopro...

1. Lipoprotein lipase (EC 3.1.1.34), which was previously shown to bind to immobilized heparin, was now found to bind also to heparan sulphate and dermatan sulphate and to some extent to chondroitin sulphate. 2. The relative binding affinities were compared by determining (a) the concentration of NaCl required to release the enzyme from polysaccharide-substituted Sepharose; (b) the concentratio...

SCHOTZ, MICHAEL C., A. SCANU AND IRVINE H. PAGE. (Cleveland Clinic Fndn. and Frank E. Bunts Educational Inst., Cleveland, Ohio.) Eject of Triton on lipoprotein lipase of rat plasma. Am. J. Physiol. 188(2): 399-402. rg57.-When postheparin plasma was incubated with a coconut oil substrate, a decrease in optical density and an increase in unesterified fatty acids occurred. The increase in unesteri...

The injection of heparin into the circulation produces a rapid increase in circulating serum lipoprotein lipase. The lipolysis system apparently circulates as a heparin-apoenzyme complex. Lipoprotein lipase activity disappears from the circulation in an exponential fashion. Available evidence suggests that a major site of removal of lipoprotein lipase activity is the liver. We have evaluated th...

Hepatic triglyceride lipase was isolated from human post-heparin plasma by the method of Ehnholm et al. using modifications which increased the specific activity 12-fold to approximately 3,000 mumol of free fatty acid/h/mg of protein. Lipoprotein lipase with similar specific activity was prepared from the same plasma samples using heparin and concanavalin A affinity chromatography. The molecula...

Thioglycolate-elicited mouse peritoneal macrophages were incubated for 24 hours in serum-free Dulbecco-Vogt medium containing 0.5% fatty acid-poor bovine serum albumin. This conditioned medium, designated MP medium, was used for experiments with bovine aortic smooth muscle cells (SMCs) or human skin fibroblasts (HSFs). Dulbecco-Vogt medium of the same albumin content but without macrophages ser...

Montero, S., Blanco, A., Virto, M. D., Landeta, L. C., Agud, I., Solozabel, R., Lascaray, J. M., De Renobles, M., Llama, M. J. and Serra, J. L. (1993) Enzyme Microb. Technol. 15,239-247 Huang, F.-C. and Ju, Y.-H. (1994) Biotechnol. Tech. 8,827-830 AIChE J. 35, 1551-1554 21 Bailie, P. M., McNerlan, S. E., Robinson, E. and Murphy, W. R. (1995) Trans. IChemE. 73, 71-76 22 Wehtje, E., Adlercreutz, ...

The effect of voluntary wheel-running to modulate sucrose-induced hypertriacylglycerolemia was investigated on both sedentary and exercised rats in relation to the timing of sucrose feeding. The exercised group was allowed voluntary wheel-running between 21.00-08.00. Rats of both groups were daily meal-fed a 35% sucrose diet at 20.00-21.00 and a basal diet at 08.00-09.00, or inversely meal fed ...