13 14 In this work, extraction and characterization of compounds with antioxidant and antimicrobial 15 activity from Haematococcus pluvialis microalga in red phase have been carried out. To do 16 this, subcritical water extraction (SWE) has been combined with analytical techniques such as 17 HPLC-DAD, HPLC-QqQMS and GC-MS and in-vitro assays (i.e., for antioxidant and 18 antimicrobial activity)...

Achyranthes aspera L. is a well known herb commonly used in traditional system of Indian medicine to treat various disorders, such as cough, dysentery, gonorrhea, piles, kidney stone, pneumonia, renal dropsy, skin eruptions, snake bite, etc. Here, we used RP-UFLC-DAD method for determining triterpenoids betulinic acid (BA), oleanolic acid (OA) and ursolic acid (UA) from A. aspera. Optimum yield...

Purpose: To determine the effects of temperature, pressure and extraction time on oil yield obtained from Polygonatum odoratum as well as the optimum processing conditions for supercritical carbon dioxide (CO2) extraction. Methods: Supercritical CO2 extraction technology was adopted in this experiment to study the process of extraction of volatile oil from Polygonatum odoratum while gas chromat...

Essential oil components were extracted from Lavandula angustifolia (Lavender) flowers using pressurized fluid extraction (PFE). A Box-Behnken design was used to optimize the effective extraction variables. The chemical composition of extracted samples was analyzed by gas chromatograph-flame ionization detector column. For achieving maximum % extraction yield the temperature, pressure, and dyna...

For accurate quantification of DNA and RNA from environmental samples, yield loss during nucleic acid purification has to be minimized. Quantitative PCR (qPCR) and reverse transcription (RT)-qPCR require a trade-off between maximizing yield and removing inhibitors. We compared DNA and RNA yield and suitability for quantitative SYBR Green PCR and RT-PCR using the UltraClean and PowerSoil extract...

This study was undertaken with the aim of establishing a correlation between the extraction yield in total flavonoids from red grape pomace and the extraction temperature, using 0.5% (w/v) aqueous lactic acid as the solvent system. Extraction of flavonoids was found to obey second-order kinetics, and on such a basis, the yield in total flavonoids at saturation could be very effectively determin...

An efficient cold-mechanical/sonic-assisted extraction technique was developed for extraction of genipin from genipap (Genipa americana) peel. Ultrasound assisted extraction (285 W, 24 kHz) was performed at 5, 10 and 15 °C for 5, 10 and 15 min. After cold-extraction, genipin was separated from pectin and proteins by aid of fungal pectinesterase. The maximum yield of non-cross-linked genipin was...

In order to study the effect of foliar applications of micronutrients on yield, extraction oil production and chemical composition of pot marigold (Calendula officinalis), two experiments were conducted in the randomized complete block design with three replications in Shahrekord, Iran during two growing seasons 2014 and 2015. The objectives of this study were to determine the effects of foliar...