background & aim: dracocephalum genus belongs to the lamiaceae family which has eight species in iran. dracocephalum kotschyi boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. in addition, this species is used in iranian traditional medicine. the aim of current study was to examine the various growth regulations and explants on direct organogenesis of d...

Acute ultraviolet light B (UVB) injury is associated with dermal mast cell histamine release. The possibility that histamine-stimulated prostaglandin (PG) synthesis could be a mechanism for irradiation erythema was therefore examined using human skin explants. Explants responded to UV irradiation (120 mJ/cm2) with a fivefold increase in synthesis of prostaglandins E2, F2 alpha and 6-keto PGF1 a...

1. Explants of mammary gland from mid-pregnant rabbits were cultured in medium 199 containing insulin, prolactin and cortisol, and specific anti-casein immunoglobulin G was used to measure the amount, rate of synthesis and rate of degradation of casein in the explants in the presence of hormones and after removal of hormones from previously stimulated tissue. 2. The amount of casein in particle...

BACKGROUND The differentiation of floor plate cells and motor neurons in the vertebrate neural tube appears to be induced by signals from the notochord. The secreted protein encoded by the Sonic hedgehog (Shh) gene is expressed by axial midline cells and can induce floor plate cells in vivo and in vitro. Motor neurons can also be induced in vitro by cells that synthesize Sonic hedgehog protein ...

Astragalus species are medicinal plants which produce valuable secondary metabolites, especially cycloartane-type glycosides. In this study, stem and leaf explants of Astragalus trojanus were subjected to different plant growth regulators, environmental conditions and media compositions to identify their callus responses. Stem and leaf explants were cultured in Murashige and Skoog (MS) and wood...

Abstract Crop genetic diversity has a significant role in improving new plants through breeding. The chrysanthemum contains the most mutant varieties, making mutation breeding one of widely utilized procedures for ornamental plants. goal this research is to use gamma irradiation induce variation and improve features. In vitro bud explants white ‘Bacardi’ type were treated with rays at 20 Gy on ...

In the present investigation, four types of explants from mature zygotic embryos coconut, viz., whole upper cotyledonary region without haustorium, half plumule with a portion radicle and exclusively plumular tissue, were cultured in 12 different media combinations to find suitable explant which could be regenerated after cryopreservation. Explants pre-cultured medium 0.4 0.5 M sucrose for thre...

Wild plant species are of great interest as a source pharmacologically valuable compounds but number them endemic and/or endangered ones. Modern biotechnology can provide reliable methods for their utilization without disturbing natural populations. In vitro culture Rhodiola being intensively developed to include into various biotechnological programmes. Aim. Development protocol direct rosea L...

Methods Explants of normal porcine vaginal mucosa (full-thickness) were infected with biofilm-producing methicillinresistant S. aureus (MRSA) (3.2x10^5 CFU) for 2h – 48h. Following infection (2h), explants were treated with the active dressing (TegadermTM CHG) for 22-46h or left untreated (controls). Formation of MRSA biofilm was evaluated by scanning electron microscopy. Also, bacteria were en...