in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

objective: to study the effects of different matrices and co-culture systems on cultured limbal stem cells (lscs). materials and methods: limbal explants were co-cultured with limbal fibroblasts (lf) and/or mouse embryonic fibroblasts (mef) on filter inserts coated with amniotic membrane (am), matrigel (mat) and collagen type i (col). results: this study revealed that am facilitated the cell mi...

many investigators are interested in finding the new cultural systems that can support the in vitro development of pre-implantation embryos better. previous studies suggested that growth factors such as epidermal growth factor (egf) are important in pre-implantation embryo development and implantation process. on the other hand, it is very important to support post thaw development of frozen em...

this study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°c in dark conditions. the results showed that different treatments had a significant effect on the germination percentage. the...

Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenh...

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...