Mice were injected intravenously with Candida albicans blastospores to establish chronic renal infection. Seventy-five percent of the animals inoculated with 10(6) blastospores died as a consequence of infection during the subsequent 60 days of observation. Intraperitoneal administration of 10 mU of partially purified human myeloperoxidase 1 day after injection of the pathogen increased surviva...

Diets containing low-quality durum wheat (716 g/kg) for production of pasta were used to feed male broiler chicks. The efficacy of xylanase supplementation and the impact of xylanase inhibitors on losses in exogenous enzyme activity were analyzed. Birds fed on the basal diet, not supplemented with recombinant xylanases or Roxazyme G, reached a BW of 1,509 g with a feed conversion ratio of 1.77 ...

Introduction Enzyme-replacement therapy (ERT) with alglucosidase alfa has improved the lifespan of patients with classic infantile Pompe disease, although ERT is not effective in a subset of patients who mount an immune response to the exogenous enzyme. We studied the development of antibodies in response to ERT and its effect on clinical outcomes in 11 patients with classic infantile Pompe dis...

Upon detergent or hypo-osmotic lysis of CHO-cell postnuclear supernatants or isolated lysosomes at pH 4.8, the lysosomal enzymes beta-hexosaminidase, beta-galactosidase, alpha-fucosidase and cathepsin C were readily pelleted, whereas the exogenous marker, long-term-internalized horseradish peroxidase, was not. Salt or pH elevation greatly decreased lysosomal-enzyme pelletability. The results su...

Homogeneous preparations of the potassium-requiring yeast aldehyde dehydrogenase are active with either DPN or TPN in the oxidation of a wide variety of aldehydes. Exogenous mercaptan is not a requirement for catalytic activity. The enzyme is denatured in 6 M guanidine hydrochloride to form a protein species with a molecular weight somewhat greater than 21,000. The denatured protein may be reas...

The hydrolytic potential of cellulases produced by Trichoderma viride, Trichoderma pseudokoningii, Trichoderma koningii, and Trichoderma reesei with addition of exogenous β-glucosidase was evaluated on cellulose of steam-pretreated Lespedeza. The T. viride enzyme achieved the highest glucose conversion (90.09%), while T. pseudokoningii cellulase achieved the highest ratio of cellobiose to gluco...

The mechanism by which the angiotensin I (AI) converting enzyme inhibitor SQ20,881 (<GluTrp-Pro-Arg-Pro-Gln-Ile-Pro-Pro) blocks the pressor response to exogenous AI was studied in mo in the intact anesthetized dog. When administered as a single dose 250 times that of Injected AI (250 nmoles/kg) into either the pulmonary or systemic circulation, SQ20,881 produced inhibition of pulmonary conversi...

Rapid synchronized seed germination is desirable to ensure seedling establishment and improve crop yield. However, abiotic stresses from drought, salinity, heavy metals have a negative impact on germination. The application of silicon (Si) has emerged as promising approach for improving germination, especially under unfavorable conditions. the mechanisms Si action not been systematically studie...

An experiment was conducted to compare a commercial corn-soybean meal diet with a pearl millet diet containing less soybean meal (-27%), alone or in combination with exogenous enzymes, on growth performance, jejunal villus development, ileal CP, and AA digestibility, and cecal microbial populations in broilers. One hundred sixty 1-d-old male Ross 508 broilers (5/cage) were randomly allocated to...