The aim of this study was to identify cancer stem cells (CSC) from three hepatocellular carcinoma (HCC) cell lines and to screen for specific microRNAs (miRNAs) regulating CSCs. Side population (SP) phenotype analysis was used. Four factors in the staining process, the incubation time, shaking interval, culture time and Hoechst 33342 concentration were explored, respectively, to define the SP s...

Twenty four bacterial strains from four different regions of mud volcano and lime cave were isolated to estimate their diversity, plant growth promoting and biocontrol activities to use them as inoculant strains in the fields. An excellent antagonistic effect against four plant pathogens and plant growth promoting properties such as IAA production, HCN production, phosphate solubilization, side...

1Department of Neurology, Universidade Metropolitana de Santos, Santos SP, Brazil; 2Multiple Sclerosis Reference Center for the Coastal Region of the State of São Paulo, Santos SP, Brazil. Correspondence: Yara Dadalti Fragoso; Departamento de Neurologia, Universidade Metropolitana de Santos; Rua da Constituição 374; 11015-470 Santos SP Brasil; E-mail: [email protected] Conflict of interest: The...

The repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. We further explored this repertoire using PCR-sequencing and culture in seven individuals living in four tropical countries. A total of 41 microeukaryotes including 38 different fungal species and three protists were detected. Four fungal species, Davidiell...

The Australian species of the ant genus Dolichoderus are revised. The fauna contains 27 species belonging to four species goups, the australis group (5 species, 2 newly described), the doriae group (6 species, 2 newly described), the scabridus poup (6 species, 1 newly described) and the scrobiculatus group (10 species, 4 newly described). The former subspecies niger Crawley and rufotibialis Cla...

A novel microbial esterase, EaEST, from a psychrophilic bacterium Exiguobacterium antarcticum B7, was identified and characterized. To our knowledge, this is the first report describing structural analysis and biochemical characterization of an esterase isolated from the genus Exiguobacterium. Crystal structure of EaEST, determined at a resolution of 1.9 Å, showed that the enzyme has a canonica...

We studied the electrophysiological characteristics of systolic (SP) and diastolic (DP) potentials recorded during sinus rhythm (SR) in the left interventricular septum of a 27 year-old woman presenting with verapamil-sensitive idiopathic left ventricular tachycardia (VT). During SR, and during VT, SP was activated from ventricular base-to-apex, and DP from apex-to-base. SP and DP were both det...

We carried out a study to investigate the presence of some protozoan parasites (Cryptosporidium sp., Giardia sp., Toxoplasma gondii) on three species of Antarctic penguins: Adélie (Pygoscelis adeliae), gentoo (Pygoscelis papua) and chinstrap (Pygoscelis antarctica) from different locations along the Antarctic Peninsula and the South Shetland Islands. Swabs and faeces samples were analysed by PC...

tion of peste des petits ruminants virus and prospects for improved diagnosis and control.dence of peste des petits ruminants (PPR) virus circulation in Algeria (Sahrawi territories): outbreak investigation and virus lineage identification. To the Editor: The genus Exigu-obacterium was first described in 1983 by Collins et al., who characterized the species E. aurantiacum (1). Since then, 9 new...