The aim of this study was to screen fish farm water and fishes reared in farms within Abakaliki metropolis for multi-drug resistant Escherichia coli. Exactly 30 samples were obtained from 10 different using sterile bottles January June, 2018. Samples analyzed by standard microbiology methods. Susceptibility test antibiotics done Kirby-Bauer disc diffusion technique. Double-disc synergy used iso...

To determine the prevalence of bacterial mastitis in cattle, milk samples positive for California mastitistest (CMT) were cultured during a period of almost 4 years. The bacterial species isolated from 2904 milksamples studied were coagulase negative Staphylococcus sp. in 879 (30.27%) samples, Streptococcusagalactiae in 642 (22.11%), S. dysgalactiae in 332 (11.43%), E. coli in 295 (10.16%), Sta...

Preparations of RNA polymerase (E.C.2.7.7.6) from uninfected Escherichia coli, T4 infected Escherichia coli, and Acinetobacter calcoaceticus when centrifuged in sucrose gradients in the absence of magnesium ions gave rise to five peaks, all of which were able to form polymers from ribonucleoside 5'-triphosphates in the absence of template or primer. All of the peaks obtained from the Escherich...

Chymosin (Rennin EC 3.4.23.4), an aspartyl proteinase, is the major proteolytic enzyme in the fourthstomach of the unweaned calf, and it is formed by proteolytic activation of its zymogene, prochymosin.Following the cloning of synthesized cDNAs on mRNA pools extracted from the mucosa of the calf fourthstomach, we have identified an alternatively spliced form of preprochymosin ...

Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the formation of insoluble inclusion bodies is a disruptive factor in this process. Various solubilization and refolding methods can be used to impro...

e live with trillions of microbes in our gut, a rich community of bacteria and other microorganisms that metabolize foods and support our health. A new animal study published in Science now suggests that gut inflammation alters this microbial community – our microbiota – fostering the growth of a toxic form of bacteria that may then cause colorectal cancer. The research also linked this toxic b...

Escherichia coli has had a central place in water microbiology for decades as an indicator of faecal pollution. It is only relatively recently that the role of E. coli as pathogen, rather than indicator, in drinking water has begun to be stressed. Interest in the role of E. coli as a cause of diarrhoeal disease has increased because of the emergence of E. coli O157:H7 and other enterohaemorrhag...

The cDNA from the mycoparasitic fungus Trichoderma atroviride PTCC5220 encoding a 42 kDa chitinase (Chit42) was isolated. The nucleotide sequence of the cDNA fragment as having a 1263 bp open reading frame that encodes a 421 amino acid polypeptide, and a high homology was found withother reported Chit42 belonging to the Trichoderma sp. The 22 amino acid N-terminal sequence is a putative s...

cloning and expression of the l-phenylalanine dehydrogenase gene, from b. sphaericus in e. coli were done. the gene was cloned in the vector pet16b and transformed into e. coli bl21 (de3). the functional form of the l-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, re...