Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

Dormant seeds from Fraxinus species require cold-temperature after-ripening prior to germination. Earlier, we found that abscisic acid (ABA) will inhibit germination of excised nondormant embryos and that this can be reversed with a combination of gibberellic acid and kinetin. Using Milborrow's quantitative "racemate dilution" method the ABA concentration in 3 types of Fraxinus seed and pericar...

embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...

S eeds are plant spores—desiccated, dormant cells in which metabolism and growth have been drastically slowed, so that the organism can wait out adverse conditions. This is especially important for plants, which cannot run away from environmental threats. Instead of motility, they use dormancy to outlast adversarial conditions caused by pathogens or severe weather. Indeed, some seeds remain via...

to produce embryonic callus in four grapevine cultivars i.e. yaghouti, bidane sefid, shahroodi and flame seedless, ovary explants were collected at flower developmental stages iii and v and cultured in ms medium supplemented with various concentrations of 2,4-d and bap. for differentiation of somatic embryos, ms medium was supplemented with various concentrations of iaa and bap. results showed ...

Germination is a critical process in the reproduction and propagation of flowering plants, also key stage industrial grain malting. commences when seeds are steeped water, followed by degradation endosperm cell walls, enzymatic digestion starch proteins to provide nutrients for growing plant, emergence radicle from seed. Dormancy state where fail germinate upon steeping, but which prevents inap...

Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...