Wax esters are major constituents of the surface lipids in many terrestrial arthropods, but their study is complicated by their diversity. We developed a procedure for quantifying isomers in mixtures of straight-chain saturated and unsaturated wax esters having the same molecular weights, using single-ion monitoring of the total ion current data from gas chromatography-mass spectrometry. We exa...

Abstract We describe a method for cleaning leaf epicuticular wax and contaminants from herbarium specimens in preparation scanning electron microscopy. Specimens are rinsed chloroform, heated at 50°C 8 hrs, ultrasonicated 70% ethanol, subjected to critical-point drying. This helps preserve reveal the surface details of that heavily covered with contaminants, making many morphologically function...

Abstract Background Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors environment. In wheat, EW associated with resilience to heat drought stress, however, current limitations on phenotyping restrict integration this secondary trait into wheat breeding pipelines. study we evaluated use light reflectance as a proxy load developed an ef...

Nine people who ate a fish curry from a mobile canteen experienced increased heart rate, flushed skin, headache, nausea and diarrhoea shortly afterwards. These symptoms, which lasted for a mean of nine hours, were thought to have been associated with a combination of fish histamine and wax ester poisoning. The incriminated fish used was eventually identified as castor oil fish (Ruvettus pretios...

Abstract Key message We identified two poplar clones of the same species as highly comparable, yet further genus to be distinctly different regarding multiple morphological and ecophysiological traits. Leaf morphology, wax composition, residual (cuticular) transpiration four (two hybrid P. × canescens , trichocarpa euphratica ) were monitored from beginning end growing season 2020. A pronounced...