Earlier works on oxidation of sulf ides, thiols, and disulf ides with both chemical and enzymic systems are reviewed. Enzymic oxidations of thiols and disulfides are compared with their chemical oxidations with N2O and H2O2 by the use of unsymmetrical disulf ides, thiolsulfinates and O18_ tracer technique. In both enzymic oxidation and that with N2O, oxidation of disulfides to thiolsulfinates i...

Monoaryl phosphonate esters, designated as analogs of the transition state in the hydrolysis of carboxylic esters, were synthesized and used as haptens to generate specific monoclonal antibodies. Some of these antibodies react with cognate aryl carboxylic esters to release a fluorescent alcohol. The reaction appears to be stoichiometric; however, the activity is slowly regenerated under alkalin...

t I AHE method most generally employed for the cytochemical localization JL of alkaline phosphatase is that due to. Takamatsu (1939) and Gomori (1939). It depends upon the hydrolysis by the enzyme of jS-glycerophosphate in the presence of calcium nitrate. Calcium phosphate is thus precipitated at the site of enzymic activity, and may be rendered visible by various procedures. The method is unsu...

The enzymic properties of alkaline phosphatase (EC 3.1.3.1) from pig kidney brush-border membranes were studied. 1. It hydrolyses ortho- and pyro-phosphate esters, the rate limiting step (V(max.)) being independent of the substrate. It transphosphorylates to Tris at concentrations above 0.1m-Tris. 2. The pH optimum for hydrolysis was between 9.8 and 10. The pK of the enzyme-substrate complex is...

A previously described technique [Rose, Simona, Offord, Prior, Otto & Thatcher (1983) Biochem. J. 215, 273-277] permits the identification of the C-terminal peptide of a protein as the only peptide that does not incorporate any 18O upon partial enzymic hydrolysis in 18O-labelled water. Formation of chemical derivatives followed by combined g.l.c.-m.s. was used in this earlier work. We now descr...