CONXENTS LIFE CYCLES ......................................................... 151 RECOMBINATION ....................................................... 152 Tetrad and random spore analysis; procedures ............................ 152 Chromosome maps ................................................... 153 Interference, chiasma, and chromatid .................................... 153 Gene convers...

We proposed "single-molecule immunoassay", quantification of single protein molecules, by developing a nanofluidics-based enzyme-linked immunosorbent assay (ELISA) device. Extremely small volume(pL-aL) and high surface-to-volume ratio of nanochannels enables ~100% capture of analyte molecules. Enzymatic amplification reaction and differential interference contrast thermal lens microscope (DIC-T...

The biosynthetic intermediate PRPP (phosphoribosylpyrophosphate) has a central role in cellular biochemistry since it links carbon and nitrogen metabolism. Its importance may be reflected in the fact that, in the Saccharomyces cerevisiae (yeast) genome, there are five unlinked genes, PRS1-PRS5, each of which is theoretically capable of encoding the enzyme synthesizing PRPP. Interference with th...

Conditions based on previous assays with potassium p-acetylphenyl sulphate have been established for the specific assay of arylsulphatase C in rat tissues. The enzyme has optimum activity with 40mm substrate at pH8.0 in the presence of 0.1m-phosphate buffer. Under these conditions arylsulphatase C can be assayed without interference from the other arylsulphatase enzymes present and is useful as...

A facile hydrothermal route for the synthesis of ordered NiWO4 nanocrystals, which show promising applications as high performance non-enzymatic glucose sensor is reported. The NiWO4-modified electrodes showed excellent sensitivity (269.6 μA mM(-1 )cm(-2)) and low detection limit (0.18 μM) for detection of glucose with desirable selectivity, stability, and tolerance to interference, rendering t...

An enzyme linked immunosorbent assay (ELISA) to measure free serum IgA antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis is described. Results were reproducible and there was no interference from crossreacting antibodies directed against other Gram negative bacteria. Titres were high in patients with Pseudomonas aeruginosa infection and differed according to the stage of the...

The chitin synthase B gene is a key enzyme in the synthesis of insect peritrophic matrix (PM), which affects insects’ feeding behavior. was cloned from transcription library Heortia vitessoides Moore. RT-qPCR showed that HvChsb highly expressed larval stage H. vitessoides, especially on first day pre-pupal stage, as well midgut larvae and abdomen adults. After starvation treatment, found to be ...

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

RNA interference-mediated silencing of the key flavonoid and isoflavone biosynthesis enzyme, respectively, by two different research groups has provided direct genetic evidence for the essential roles that these compounds play in nodulation. Anton Wasson et al. have shown that flavonoids are essential for localized auxin transport inhibition during nodulation in the indeterminate legume Medicag...