Protein immobilization on solid interfaces is a crucial aspect of their successful application in technologies such as biosensing, purification, separation, decontamination, etc. Although immobilization can improve the long-term and operational stability of proteins, this is often at the cost of significant losses in the catalytic activity of the tethered enzyme. Covalent attachment methods tak...

Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES-NH2) were successfully synthesized as novel materials for immobilization. Membranes with various po...

Immobilization has emerged since last decade as a very powerful tool to improve almost all enzyme properties like stability, activity, specificity and selectivity, and reduction of inhibition. The immobilization may help to solve some of the problems of enzymes as industrial biocatalysts like enzyme recovery for reuse. In the present investigation A.niger was isolated from soil and identified b...

Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES–NH2) were successfully synthesized as novel materials for immobilization. Membranes with various po...

A glucoamylase-immobilized system based on cross-linked gelatin nanoparticles (CLGNs) was prepared by coacervation method. This system exhibited characteristics of temperature-triggered phase transition, which could be used for enzyme immobilization and release. Their morphology and size distribution were examined by transmission electron microscopy and dynamic light scattering particle size an...

Enzymatic catalysis has been pursued extensively in a wide range of important chemical processes for their unparalleled selectivity and mild reaction conditions. However, enzymes are usually costly and easy to inactivate in their free forms. Immobilization is the key to optimizing the in-service performance of an enzyme in industrial processes, particularly in the field of non-aqueous phase cat...

Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papa...

Citation: Castro-Muñiz A, Hoshikawa Y, Komiyama H, Nakayama W, Itoh T and Kyotani T (2016) Improving the Direct Electron Transfer in Monolithic Bioelectrodes Prepared by Immobilization of FDH Enzyme on Carbon-Coated Anodic Aluminum Oxide Films. Front. Mater. 3:7. doi: 10.3389/fmats.2016.00007 improving the Direct electron Transfer in Monolithic Bioelectrodes Prepared by immobilization of FDh en...

An isolated strain (from rice), Bacillus acidocaldarius was able to produce extracellular α-amylase.The enzyme was partially purified with 50% acetone and showed 4.3-fold purification. Amylase was immobilized on different carriers by different methods and its specific activity for starch hydrolysis studied. Immobilized α -amylase on glass beads (covalent binding) and cation exchange resin (ioni...

In this study, zinc oxide (ZnO) nanocrystals with different morphologies were synthesized and used as substrates for enzyme immobilization. The effects of morphology of ZnO nanocrystals on enzyme immobilization and their catalytic activities were investigated. The ZnO nanocrystals were prepared through a hydrothermal procedure using tetramethylammonium hydroxide as a mineralizing agent. The con...