Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing digestibility of RS-3 are largely unknown. This research aims to reveal how crystal and molecular weight (distribution) influence its resistance. Narrow polydisperse ?-glucans degree polymerization (DP) 14–76, either obtained enzymatic ...

With an ultra-high surface area and abundant functional groups, graphene oxide (GO) provides an ideal substrate for the immobilization of trypsin. We demonstrated that trypsin could be immobilized on GO sheets assisted by polymers as molecular spacers to maintain the activity of the enzyme. And with the trypsin-linked GO as the enzyme immobilization probe, a novel microwave-assisted on-plate di...

The use of thermal denaturation of proteins prior to in-solution digestion and mass spectral peptide mass mapping is reported. Thermal denaturation is preferred over chemical denaturation because it does not require purification/concentration prior to mass spectral analysis. Enzymatic digestions of proteins that are resistant to proteolysis are significantly enhanced by thermal denaturation. Na...

In vitro methods did not accurately predict in vivo apparent total tract digestibility of energy for canola coproducts (r = -0.81; P = 0.002). We scanned 153 samples of digesta, feces, and in vitro digestion residues (ivR) on a Fourier transform midinfrared instrument with a single-reflection attenuated total reflectance attachment. The second derivative spectral net intensities of the carbonyl...

Transfer ribonucleic acids (tRNAs) are challenging to identify and quantify from unseparated mixtures. Our lab previously developed the signature digestion approach for identifying tRNAs without specific separation. Here we describe the combination of relative quantification via enzyme-mediated isotope labeling with this signature digestion approach for the relative quantification of tRNAs. The...

Abstract In this study, the bioaccessible total phenolic compounds (TPC) and antioxidant capacity (AC) of commonly consumed bakery products enriched with raw (BH) or roasted (RBH) buckwheat hull were evaluated. The soluble insoluble fractions obtained after in vitro enzymatic digestion mixed rye/wheat bread 4% RBH (BRBH), wheat 3% BH (BBH), their control counterparts separated (C-BRBH C-BBH, re...

Background: Bioactive compounds need to resist food processing, be released from the matrix, and bioaccessible in gastrointestinal tract order provide health benefits. isolated peach pomace (PP) were encapsulated using four different wall materials improve their stability evaluate effects of vitro digestion, as well chemometric modeling among obtained encapsulates. Methods: Phenolics carotenoid...