نتایج جستجو برای: enzymatic degumming
تعداد نتایج: 55111 فیلتر نتایج به سال:
Simulations and modelling [e.g. with combined QM/MM (quantum mechanics/molecular mechanics) methods] are increasingly important in investigations of enzyme-catalysed reaction mechanisms. Calculations offer the potential of uniquely detailed, atomic-level insight into the fundamental processes of biological catalysis. Highly accurate methods promise quantitative comparison with experiments, and ...
BACKGROUND Chemistry and particularly enzymology at surfaces is a topic of rapidly growing interest, both in terms of its role in biological systems and its application in biocatalysis. Existing protein immobilization approaches, including noncovalent or covalent attachments to solid supports, have difficulties in controlling protein orientation, reducing nonspecific absorption and preventing p...
This study offers a detailed mechanistic investigation of host-guest encapsulation behavior in a new enzyme-mimetic metal-ligand host and provides the first observation of a conformational selection mechanism (as opposed to induced fit) in a supramolecular system. The Ga4L4 host described features a C3-symmetric ligand motif with meta-substituted phenyl spacers, which enables the host to initia...
Mechanistic models for biochemical systems are frequently proposed from structural data. Site-directed mutagenesis can be used to test the importance of proposed functional sites, but these data do not necessarily indicate how these sites contribute to function. In this study, we applied an alternative approach to the catalytic mechanism of alkaline phosphatase (AP), a widely studied prototypic...
Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix...
Reflections on advances and retreats in biochemistry have, over the years, generated several maxims. The late Efraim Racker’s ‘don’t waste clean thinking on dirty enzymes’ has been one of the most durable. On the occasion of a lecture in Jerusalem a decade ago, I framed a list of the ten ‘best’ maxims as ‘commandments’, which were later published as a ‘Guest Commentary’ in the Journal of Bacter...
This paper focuses on the effect of okra bast fillers biodegradation poly(vinyl alcohol) composites. Fibers were obtained from plant stems via biological degumming and subjected to different chemical treatments such as scouring, alkalizing, maleic anhydride treatment, vinyl acetate grafting. The fibers ground PVA-okra filler composites produced at 20 wt% fiber load solution casting method. test...
Due to its large number of potential utilizations Jatropha curcas L. (physic nut, purging nut), a tropical plant cultivated in many Latin American, Asian and African countries, has become topic of various research projects. Nutritional as well as technical applications, however, are restricted due to the plant’s toxicity. The seed oil contains phorbol esters, a family of compounds known to caus...
Polygalacturonases (PGases) or hydrolytic depolymerases are enzymes involved in the degradation of pectic substances. They have a wide range of applications in food and textile processing, degumming of plant rough fibres and treatment of pectic wastewaters. Bacteria, yeasts and fungi under both submerged (SmF) and solid-state fermentation (SSF) conditions produce these enzymes. Bacteria produce...
This paper carried out experimental study, through extraction and characterization of both crude and refined castor oil. Normal hexane was used as solvent for the extraction process. The oil produced was refined through degumming, neutralization and bleaching process using local adsorbent (activated clay). The characterization analysis revealed that tested parameters, which include specific gra...
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