l-asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.in this study, simple and advantageous method has been validated for rapid and precise determination of intracellular l-asparaginasein bacterial species. a suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

Fruit industries require convenient peeling method, especially during puree processing to prevent deterioration of fruit quality and product loss. Therefore, manual, chemical (sodium hydroxide/NaOH) and enzymatic (Pectinex Ultra SP-L) peeling methods were compared to determine the peeling efficiencies of ‘Chok Anan’ mangoes. The effect of different peeling parameters (concentrations [chemical p...

Motivation: Availability of large volumes of genomic and enzymatic data for taxonomically and phenotypically diverse organisms enables researchers to explore the adaptive mechanisms that led to diversification of enzymatic functions. We present Chisel, a computational framework and a pipeline for an automated, high-resolution analysis of evolutionary variations of enzymes. Chisel allows automat...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewa...

a long-term study was conducted between 1985 and 2003 on rehabilitation trials at sishen ironore mine, south africa, to identify grass species that would survive in the artificial growth medium appliedto the sites, and to determine the most suitable medium for sustainable vegetation growth. vegetationestablishment was tested at slopes of 18° and 34° and with five different cover materials. afte...

gelatin is a protein hydrocolloid. because of its unique characteristics such as gel forming, thickening, stabilizing, emulsifiing, foam and film forming, is widely used in food industries. in this research, in order to optimize the factors which affect enzymatic extraction method of gelatin ,the effects of three variables, enzyme concentrations(2-10 ppm), time(8-16 hours) and temperature(60-80...

abstract          background and objectives: concentration low-density lipoprotein (ldl) is one of the strongest indicators of atherosclerosis and predicts the diagnosis of cardiovascular diseases. ldl measurement accuracy is very important. ldl can be measured directly, such as enzymatic and nephelometry methods or can be calculated using friedewald's formula. despite the development of e...

ERK2 are protein kinases that during the enzymatic catalysis, in contrast to traditional enzymes, utilize additional interactions with substrates outside of the active sites. It is widely believed that these docking interactions outside of the enzymatic pockets enhance the specificity of these proteins. However, the molecular mechanisms of how the docking interactions affect the catalysis remai...

Agricultural feedstocks (brewers' spent grain - BSG, corncob - CC, corn husk - CH, wheat straw - WS and Luffa sponge - LS) were pretreated by liquid hot water (LHW) in order to increase cellulose recovery and enzymatic saccharification. LHW-pretreatment resulted in hemicellulose solubilization, and solids enriched in cellulose. Chemical analysis showed different susceptibilities of the feedstoc...

Enzymatic inhibition is one of the key regulatory mechanisms in cellular metabolism, especially enzymatic competitive by product. This process helps cell regulate activities. In this paper, we derive a mathematical model describing The consists coupled system nonlinear ordinary differential equations for species interest. Using nondimensionalization analysis, formula product formation rate mech...