Enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (SSCs) transplantation. Knock-out serum replacement (KSR) has been reported to enhance the proliferation of murine SSCs and human embryonic stem cells. The aim of the present study was to investigate the effect of KSR versus fetal bovine serum (FBS) ...

Listeria monocytogenes is considered as a potential live bacterial vector, particularly for the induction of CD8 T cells. The CD4 T-cell immune response triggered after enteral immunization of mice has not yet been thoroughly characterized. The dynamics of gamma interferon (IFN-gamma)- and interleukin-4 (IL-4)-secreting CD4 T cells were analyzed after priming through intragastric delivery of an...

BACKGROUND A retrospective utilization study was performed to evaluate utilization patterns for enteral nutrition in a university teaching hospital. METHODS Enteral nutrition was divided into three types according to the nitrogen source, ie, total protein type [Nutrison Fibre(®), Fresubin Energy Fibre(®), Fresubin(®), Supportan(®) (a special immunonutrition for cancer patients or patients wit...

OBJECTIVE To determine the factors that influence the adequacy of enteral nutritional therapy in an intensive care unit. METHODS This prospective observational study was conducted in an intensive care unit between 2010 and 2012. Patients >18 years of age underwent exclusive enteral nutritional therapy for ≥72 hours. The energy and protein requirements were calculated according to the ICU prot...

INTRODUCTION AND AIMS Microbial contamination of enteral feeding and infant formulas can result in a risk of worsening of the clinical condition of the patients, who are already weakened and susceptible to pathogens. The objective was to evaluate aspects of the management of quality hygienic - sanitary of enteral feeding and infant formulas in hospitals, focusing on the structure, process and o...

Specialized nutrition support should be offered to patients who are malnourished or at risk of becoming malnourished when it would benefit patient outcomes or quality of life. Improving the nutritional value of ingested food and tailoring intake to the patient's preferences, abilities, and schedule should be the first measures in addressing nutritional needs. When these interventions alone are ...

BACKGROUND It is believed that certain nutrients such as glutamine, arginine and omega-3 fatty acids may play a significant role in metabolic, inflammatory, and immune processes in acute pancreatitis. The present systematic review aimed to define whether the addition of these substances to enteral nutrition provides any clinical benefit over standard enteral formulas in patients with acute panc...

In surgery, indications for artificial nutrition comprise prevention and treatment of catabolism and malnutrition. Thus in general, food intake should not be interrupted postoperatively and the re-establishing of oral (e.g. after anastomosis of the colon and rectum, kidney transplantation) or enteral food intake (e.g. after an anastomosis in the upper gastrointestinal tract, liver transplantati...

Nutritional therapy in the critically ill patient in the intensive care unit (ICU) is an important part of the total care of the patient. Nutrition can be given through parenteral nutrition or enteral nutrition therapy. Parenteral nutrition has been limited recently, but not exclusively, to patients who, for a variety of reasons, cannot be fed enterally. Although the debate concerning parentera...