the chambers. (ii) A drop of the culture is placed on the grid and then withdrawn until the fluid level is just below the surface of the grid; if the surface is wet, the covering film will not stick properly. (iii) The floor of the chamber is formed by dipping the cover slip through a film which has been previously cast on glass and stripped off onto a water surface; casting, stripping, and dip...

A novel virus lytic for Pseudomonas aeruginosa has been purified. Its viral particles have a siphoviral morphology with head 60 nm in diameter and noncontractile tail 184 long. The dsDNA genome consists of 16,449 bp, cohesive 3′ termini, encodes 28 putative proteins single strain. peptidoglycan endopeptidase encoded by ORF 16 was found to be the enzyme this virus. recombinant, purified active u...

The increasing spread of antibiotic-resistant pathogens has raised the interest in alternative antimicrobial treatments. In our study, the functionally active gram-negative bacterium bacteriophage CP933 endolysin was produced in Nicotiana benthamiana plants by a combination of transient expression and vacuole targeting strategies, and its antimicrobial activity was investigated. Expression of t...