Cellulase is a group of enzymes (endoglucanase, exoglucanase and betaglucosidase) required for cellulosic feedstock hydrolysis during bioethanol production. The use of recombinant cellulase is a strategy to reduce the enzyme cost. In this context, the present work describes the construction of a cellulase expression vector (pEglABglA), which allowed constitutive co-expression of endoglucanase A...

This article reports the characterization of the Clostridium thermocellum SdbA protein thought to anchor the cellulosome to the bacterial cell surface. The NH2-terminal region of SdbA consists of a cohesin domain which specifically binds the dockerin domain of the cellulosomal scaffolding protein CipA. The COOH-terminal region consists of a triplicated segment, termed SLH repeats, which is pres...

A cross-linked mutant endoglucanase II was prepared for enzymatic polymerization to cellulose. The cross-linked enzyme is composed of three mutant enzymes showing polymerization activity. A characteristic feature of the polymerization with this cross-linked enzyme is formation of cellulose fibrils in contrast to plate-like crystals obtained by using a free enzyme.

the expression of endoglucanase gene and protein profile belonging to two fungal species, penicillium oxalicum 1sms and trichoderma viride 156ms with high cellulase enzyme activity, was investigated. fungal isolates were cultured on inducer cmc medium and then the amount of released sugar and protein were assayed every three days for a month, using arsenate molybdatereagent and bradford method,...

Abstract Background Pulp refining is an energy consuming, but integral part of paper production with the aim to increase tensile strength and smoothness sheets. Commercial enzyme formulations are used lower requirements by pre-treatment pulp before refining. However, a high number different commercial products available on market containing enzymes varying origin composition, which complicates ...