Context Genome editing enables the introduction of beneficial sequence variants into genomes animals with high genetic merit in a single generation. This can be achieved by introducing primary cells followed producing live animal from these somatic cell nuclear transfer cloning. The latter step is associated low efficiencies and developmental problems due to incorrect reprogramming donor cells,...

Homogyne alpina (L.) Cass. represents the only species of genus in Slovakia. This study characterizes H. terms chromosome counts, genome size, reproduction mode and distribution Three known cytotypes are known, which cytotype 2n = 160 is represented Slovak mountains has not been documented from other countries yet. Flow cytometric analyses showed size 2C 21.30 pg for petioles 21.82 seeds. Sexua...

Since the birth of first IVF baby, Louise Brown, in 1978, researchers and clinicians have sought ways to improve pregnancy outcomes through embryo selection. In 1990s, blastomere biopsy fluorescence situ hybridization (FISH) were developed human embryos for assessment aneuploidy translocations. Limitations number chromosomes that could be assayed with FISH lead development comparative genomic (...

BACKGROUND Although chromosomal mosaicism in human preimplantation embryos has been described for almost two decades, its exact prevalence is still unknown. The prevalence of mosaicism is important in the context of preimplantation genetic screening in which the chromosomal status of an embryo is determined by the analysis of a single cell from that embryo. METHODS Here we report a systematic...

OBJECTIVE To describe in vitro development of human embryos derived from an individual with a homozygous pathogenic variant in NLRP7 (19q13.42) and recurrent hydatidiform mole (HM), an autosomal recessive condition thought to occur secondary to an oocyte defect. METHODS A patient with five consecutive HM pregnancies was genomically evaluated via next generation sequencing followed by controll...

the use of donated embryos has offered hope for infertile couples who have no other means to have children. in iran, fertility centers use fertile couples as embryo donors. in this paper, the advantages and disadvantages of this procedure will be discussed. we conclude that embryo-donation should be performed with frozen embryos thus preventing healthy donors from being harmed by fertility drug...

OBJECTIVE Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS ...