Abstract Study question To fill gaps in the evidence base on autologous superior mitochondria donor cells, and its therapeutic effect human embryo development among infertile population. Summary answer Autologous urine-derived mesenchymal stromal cells (USC) transfer may be an effective strategy to improve embryonic development, especially females with advanced age. What is known already Mitoch...

background: it has been demonstrated that performing a mock embryo transfer (et) significantly improves the pregnancy rate in ivf cycles. the mock et could be performed prior to the stimulation cycle or immediately before real et. the problem of the first procedure is the possibility of variation in the uterine position and or cervico�uterine angle. objective: the purpose of this study was to d...

background: natural endometrium in frozen-thawed embryo transfer (fet) may have some benefits upon implantation in patients with repeated implantation failure (rif). it might be due to possible differences between natural and stimulated endometrial growth factors and cytokins secretions. objective: the objective of this study was to compare the pregnancy rate of fet on modified natural cycle ve...

Polarity is essential for generating cell diversity. The one-cell C. elegans embryo serves as a model for studying the establishment and maintenance of polarity. In the early embryo, a myosin II-dependent contraction of the cortical meshwork asymmetrically distributes the highly conserved PDZ proteins PAR-3 and PAR-6, as well as an atypical protein kinase C (PKC-3), to the anterior. The RING-fi...

Separase is a protease that promotes chromosome segregation at anaphase by cleaving cohesin. Several non-proteolytic functions of separase have been identified in other organisms. We created a transgenic C. elegans line that expresses protease-dead separase in embryos to further characterize separase function. We find that expression of protease-dead separase is dominant-negative in C. elegans ...

Paeonia ostii ‘Feng Dan’ is an economically important, multipurpose woody plant in terms of its medical, ornamental, and oil values; however, there a noticeable contradiction between the increasing demands lack excellent germplasm resources because traditional breeding propagation approaches. In vitro embryo culture attractive option for this issue. This study presents protocol immature P. Dan’...

This study aimed to evaluate the impact of a short insemination procedure on embryo development, assess the reliability of a fertilization check prior to the appearance of pronuclei and elucidate the role of rescue intracytoplasmic sperm injection (R-ICSI). Patients who had ≥9 eggs, were aged <38 years and had normal semen samples were included. The sibling eggs of each patient were divided int...