A precise embryo quality evaluation is of paramount importance to sustain a successful in vitro fertilization (IVF) program. In most IVF clinics around the world, this quality assessment relies mainly on the morphological evaluation of cleavage stage embryos. Embryologists should be able to correlate the features observed at the optical microscope with the implantation potential of each particu...

In flowering plants, two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, which is a tissue that supports embryo development. MEDEA (MEA) encodes an Arabidopsis SET domain Polycomb protein. Inheritance of a maternal loss-of-function mea allel...

Purpose The aim of the study was to evidence whether Thyrotropin (TSH) and Thyroid hormones (T3 and T4) have an impact in in vitro follicle growth of mouse preantral follicles, in in vitro fertilization and in early embryo development. Methods This prospective observational animal experimental study was conducted on a study population consisted of 100 female and 30 male mice (C57BL/6 x CBA) F1 ...

After more than a century of research, the mouse remains the goldstandard model system, for it recapitulates human development and disease and is quickly and highly tractable to genetic manipulations. Fundamental to the power and success of using amousemodel is the ability to stage embryonic mouse development accurately. Past staging systems were limited by the technologies of the day, such tha...

introduction: the purpose of this study was to investigate the effect of rpmi and t6 medium on two cell mouse embryo co-culture with vero cells. materials and methods: two cell mouse embryos (nmri strain) were flushed from the excised uteri of superovulated mice. morphologically normal embryos were divided into four groups: the first control group was cultured on t6 media. the first experimenta...

Free abscisic acid (ABA) in integuments, nucellus, endosperm, and embryo was determined throughout seed development of peach (Prunus persica L. cv Springcrest). Quantification of ABA was performed using combined high performance liquid chromatography-radioimmunoassay based on a monoclonal antibody raised against free (S)-ABA. In the integuments and endosperm, ABA concentration remained constant...

Oct-6 is a POU transcription factor that is thought to play a role in the differentiation of cells of neuroectodermal origin. To investigate whether the Oct-6 protein could play a role in the establishment of neuroectoderm in vivo we studied the expression of the Oct-6 protein during early mouse development. Expression is first observed in the primitive ectoderm of the egg cylinder stage embryo...

It has been suggested that first embryo cleavage can be related with the embryonic-abembryonic axis at blastocyst stage in mice. Thus, cells of the 2-cell embryo might be already biased to form the inner cell mass or trophectoderm. This study was conducted to observe the possible effects of embryo biopsy on cell allocation patterns during embryo preimplantation in two different mouse strains an...

Experiments were performed to determine the actions of recombinant bovine interleukin-1beta (IL-1beta) on the growth of preimplantation embryos. In the first series of studies, IL-1beta was added at 8-10 h after insemination, and the percentage of oocytes developing to the blastocyst stage was evaluated. IL-1beta increased development to the blastocyst stage when embryos were cultured at high d...