Loop-mediated isothermal amplification (LAMP), a rapid nucleic acid amplification method, was developed for the clinical diagnosis of toxoplasmosis. Three LAMP assays based on the SAG1, SAG2, and B1 genes of Toxoplasma gondii were developed. The sensitivities and specificities of the LAMP assays were evaluated by comparison with the results of conventional nested PCR. The LAMP assays were highl...

A one-year field study compared the conventional 250W IR heat lamp with an energy-efficient 175W radiant heat lamp for swine farrowing operations. The energy-efficient heat lamp showed a $36 annual cash savings per unit (assuming $0.10/kWh electricity); a 1.2% absolute reduction in piglet mortality from birth to weaning (5.0±0.28% vs. 6.2±0.44%) (P<0.01); a 45% lower lamp failure rate (18±4% vs...

Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...

In this paper will be reported the implementation of a flexible platform to study electronic ballasts systems. The platform is a microcontrolled and FPGA-based system and was developed to generate command signals to the half bridge inverter. A specific hardware using FPGA was developed to generate the switches signals command. This hardware is responsible for the autonomous frequency (FM) and p...

rhabditidae is a family of free-living nematodes. free living nematodes due to their active movement and resistance to chlorination, do not remove in conventional water treatment processes thus can be entered to distribution systems and cause adverse health effects. ultraviolet radiation (uv) can be used as a method of inactivating for these organisms. this cross sectional study was done to inv...

ABSTRACT Designing around patents is prevalent but not often appreciated as a means by which promote economic development through competition. We provide novel empirical study of the extent and timing designing patent claims. filing rate incandescent lamp-related during 1878–1898 find that enforcement Edison’s lamp in 1891–1894 stimulated surge patenting. studied specific design features lamps ...

AIMS In this study, a loop-mediated isothermal amplification (LAMP) assay has been developed and evaluated for the rapid and sensitive detection of Verticillium dahliae Kleb., the causal agent of vascular wilts in many economically important crops. METHODS AND RESULTS LAMP primers were designed based on a previously described RAPD marker, and the LAMP assay was applied for direct detection of...

OBJECTIVES Recent research suggests that lysosomal-associated membrane protein-2 (LAMP-2) could be one of the target antigens in the pathogenesis of vasculitides. We established a transgenic rat model, env-pX rats, with various vasculitides including cutaneous vasculitis. Human primary cutaneous vasculitis includes cutaneous polyarteritis nodosa (CPN) and Henoch-Schönlein purpura (HSP). We meas...

Ag processing is a critical step in defining the repertoire of epitope-specific immune responses. In the present study, HIV-1 p55Gag Ag was synthesized as a DNA plasmid with either lysosomal-associated membrane protein-1 (LAMP/gag) or human dendritic cell-LAMP (DC-LAMP/gag) and used to immunize mice. Analysis of the cellular trafficking of these two chimeras demonstrated that both molecules col...