A variant of Sindbis virus has been isolated by growing a stock of virus, previously passaged on chicken embryo cells, in mouse plasmacytoma (MOPC 315) cells in suspension culture. An indirect immunofluorescence test and infective center assay showed that only a small fraction of cells could be infected by the stock wild-type virus, but that the population of virus accumulating after a few pass...

The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF).Ten days after, primary outgrowths were mechanically dissected into several small clumps and transf...

Surgery and radiation are the current standard treatments for cervical cancer. However, there is no effective therapy for metastatic or recurrent cases, necessitating the identification of therapeutic targets. In order to create preclinical models for screening potential therapeutic targets, we established 14 patient-derived xenograft (PDX) models of cervical cancers using subrenal implantation...

Comparisons of genome sequence data between different strains and isolates of Neisseria spp., such as Neisseria gonorrhoeae, reveal that over the evolutionary history of these organisms, large scale chromosomal rearrangements have occurred. Factors within the genomes, such as repetitive sequences and prophage, are believed to have contributed to these observations. However, the timescale in whi...

monetary compensation for human eggs used in research is a controversial issue and raises major concerns about women’s health and rights, including the potential of exploitation and undue inducement. human eggs are needed for various types of studies and without payment, it would be impossible to procure sufficient eggs for vital research. therefore, a solution seems necessary to prevent exploi...

this experiment was conducted to evaluate the effects of different levels of portulaca oleracea (po),medicinal plant, on performance, egg traits, blood biochemical and immunity parameters of laying hens. one hundred eighty hi-line (w-36 strain) mature laying hens 65 up to 76 weeks of age were allotted in a completely randomized design consisting of 5 treatments and 3 replicates (12 birds per re...

In Cloud computing the user requests are passaged to data centers (DCs) accommodate resources. It is essential select suitable DCs as per so that other should not be penalized in terms of time and cost. The searching strategies consider execution rather than related penalties while DCs. this work, we discuss Penalty Elimination-based DC Allocation (PE-DCA) using Guided Local Search (GLS) mechan...