Background Despite its importance as a widely-planted crop tree, eucalypt species and hybrids are relatively difficult to micropropagate, culture and genetically transform in vitro. Compared to other plant species, few non-commercial laboratories are proficient at Eucalyptus tissue culture and transformation. We have undertaken to establish and transform several eucalypt clones in the laborator...

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (X.Z., F.Q., W.L., Y.X., L.X., W.Y., J.Y.), College of Engineering (C.H., D.W., L.D., K.W., W.Y.), and Ministry of Agriculture Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River (G.C.), Huazhong Agricultural University, Wuhan 430070, People’s Republic of Ch...