A putative ABC transporter, fit, with significant homology to several bacterial iron transporters was identified in Escherichia coli. The E. coli fit system consists of six genes designated fitA, -B, -C, -D, -E, and -R. Based on DNA sequence analysis, fit encodes an outer membrane protein (FitA), a periplasmic binding protein (FitE), two permease proteins (FitC and -D), an ATPase (FitB), and a ...

Background: Enantiopure epoxides are important intermediates in the synthesis of high-value chiral chemicals. Epoxide hydrolases have been exploited in biocatalysis for kinetic resolution of racemic epoxides to produce enantiopure epoxides and vicinal diols. It is necessary to obtain sufficient stable epoxide hydrolases with high enantioselectivity to meet the requirements of i...

four exons of the human genomic gm-csf gene were assembled together using gene splicing by overlap extension (soe) method. the resulting nucleotide sequence was cloned in the pet23a(+) ex pression vector under the control of strong bacteriophage t7 transcription and translation signals. the construct obtained was transferred into the e. coli strain, bl21(de3) plyss and iptg was used for inducti...

objective: bone morphogenetic protein-7 (bmp-7) is a multifunctional growth factor predominantly recognized for its osteoinductive properties. due to the high cost of this protein, the availability of bmp-7 for treatment is limited. the heterologous production of recombinant hbmp-7 has been performed in a number of expression systems. in this study a novel form of bmp-7 was expressed in eukaryo...

Cell-free environments are becoming viable alternatives for implementing biological networks in synthetic biology. The reconstituted cell-free expression system (PURE) allows characterization of genetic networks under defined conditions but its applicability to native bacterial promoters and endogenous genetic networks is limited due to the poor transcription rate of Escherichia coli RNA polyme...

BACKGROUND AND OBJECTIVES Baculovirus can be used as a vector in gene delivery system. Viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system. In this regard, the gene coding for A subunit of shiga toxin (StxA) from Escherichia coli (E. coli) strain was cloned in a baculovirus expression system. StxA subunit has the abili...

Auto-inducible promoter systems have been reported to increase soluble product formation in the periplasm of E. coli compared inducer-dependent systems. In this study, we investigated phosphate (PO4)-sensitive phoA expression system (pAT) for production a recombinant model antigen-binding fragment (Fab) detail. We explored impact non-limiting and limiting PO4 conditions on strain physiology as ...

A loop deletion mutant of outer membrane protein F (OmpF) that facilitates the cellular uptake decoy molecules into Escherichia coli has been developed. In their Communication (e202111612), Osami Shoji and co-workers report co-expression OmpF with wild-type CYP102A1 enables an E. coli-based whole-cell biocatalyst to efficiently oxidise benzene, alkyl- cycloalkylbenzenes help molecules, bringing...

recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...