Purpose: To investigate the effect of Twist family basic helix-loop-helix transcription factor 1 (TWIST1) on intracranial aneurysms.Methods: A rat model aneurysm was established by ligating posterior branches both renal and left common carotid arteries. Pathological changes in arterial wall were investigated using hematoxylin-eosin staining. TWIST1 expression assessed quantitative reverse polym...

To investigate the effect and related mechanism of propofol anesthesia on pyroptosis in neonatal rat hippocampus. Neonatal rats were divided into a control group (injected intraperitoneally with saline), 80 mg/kg propofol) propofol+MCC950 10 MCC950) administered for 5 d. The day after end drug administration, hippocampi analyzed by transferase dUTP nick labeling assay interleukin beta-1, interl...

Purpose: To investigate the effect of micro-ribonucleic acid (miR)-208a on heart failure (HF) in rats through β-catenin pathway.Methods: A total 24 specific pathogen-free female Sprague-Dawley were enrolled and randomly divided into 3 equal groups, namely, control (normal group), model, study group (miR-208a), with 8 each. Echocardiography was utilized to evaluate cardiac function, terminal deo...

Staphylococcus aureus superantigen-carrying pathogenicity islands (SaPIs) play a determinant role in spreading virulence genes among bacterial populations that constitute a major health hazard. Repressor (Stl) proteins are responsible for the transcriptional regulation of pathogenicity island genes. Recently, a derepressing interaction between the repressor Stl SaPIbov1 and dUTPase from the φ11...

Numerous agents can damage the DNA of prokaryotes in the environment (e.g., reactive oxygen species, irradiation, and secondary metabolites such as antibiotics, enzymes, starvation, etc.). The large number of potential DNA-damaging agents, as well as their diverse modes of action, precludes a simple test of DNA damage based on detection of nucleic acid breakdown products. In this study, free 3'...

The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay. Cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Bile administration resulted in dose-dependent c...