in this study we estimated the allele and genotype frequencies of scd1 and dgat1 gene polymorphism. the analysis was conducted on 408 holstein cows from five dairy herds in isfahan province. genotypes were identified using pcr-rflp technique. two genetic variants (a and v) of the scd1 gene were observed in this experiment. the frequency of a-allele ranged from 0.49 to 0.66, while frequency of v...

introduction: endurance training has significant effects on the renewal of heart tissue, including myosin heavy chain (mhc) proteins. on the other side, purine-rich element-binding protein β (purb) decreases the &alphamhc; gene expression. the aim of this study was to determine the impact of exercise endurance training on purb gene expression in the heart of wistar rats. methods: fourteen rats ...

brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

We have characterized 14 human colorectal carcinoma cell lines established from primary and metastatic sites by us during the years 1982 to 1985. Five lines were established in fully defined ACL-4 medium and 9 in serum supplemented RIO medium. However, after establishment, cultures could be grown interchangeably in either medium. The lines grew as floating cell aggregates in ACL-4 medium, while...

BACKGROUND Sulawesi in Indonesia has a unique geographical profile with assumed separation from Sundaland. Studies of Helicobacter pylori in this region are rare due to the region's rural location and lack of endoscopy equipment. Indirect methods are, therefore, the most appropriate for measuring H. pylori infection in these areas; with the disposable gastric brush test, we can obtain gastric j...

Quantitation of circulating tumor cells (CTCs) can provide information on the stage of a malignancy, onset of disease progression and response to therapy. In an effort to more accurately quantitate CTCs, we have synthesized fluorescent conjugates of 2 high-affinity tumor-specific ligands (folate-AlexaFluor 488 and DUPA-FITC) that bind tumor cells >20-fold more efficiently than fluorescent antib...