Replication licensing factor (RLF) is a multiprotein complex involved in ensuring that chromosomal DNA replicates only once in a single cell cycle. It comprises two components, termed RLF-M and RLF-B. Purified RLF-M consists of a mixture of complexes containing all six members of the MCM/P1 family of minichromosome maintenance proteins. The precise composition of these different complexes and t...

INTRODUCTION Young drivers aged 17-25 continue to represent a major public health problem in Australia. Their fatality rate is approximately twice that of older-age drivers, a ratio that has remained relatively unchanged for over a quarter of a century. One response to this over-representation in fatal crashes has been the promotion of formal driver training and education, either in the pre-lic...

The views expressed are the responsibility of the authors and are not necessarily those of the Council. Abstract Speeding is commonly recognised as problem behaviour among young novice drivers and a major risk factor for their involvement in crashes. This study undertook a descriptive analysis of the speeding offences and demerit points incurred by a sample cohort of 1,277 young West Australian...

Individuals who fall asleep at the wheel usually do so because they are sleep deprived. It is likely that they are aware of the circumstances leading to sleepiness and of feeling sleepy before the event. Nevertheless, sleepiness sufficient to cause or contribute to an accident may involve a disorder of sleep, and little attention has been given to such disorders in the consideration of accident...

Replication licensing factor (RLF) is involved in preventing re-replication of chromosomal DNA in a single cell cycle, and previously has been separated into two components termed RLF-M and RLF-B. Here we show that Xenopus RLF-M consists of all six members of the MCM/P1 protein family, XMcm2-XMcm7. The six MCM/P1 polypeptides co-eluted on glycerol gradients and gel filtration as complexes with ...