The role of extracellular Ca2+ in toxic liver injury was examined in two in vitro models of the rat liver, the isolated perfused liver and isolated hepatocytes. The toxins t-butylhydroperoxide and carbon tetrachloride, as well as the Ca2+ ionophore, heptafluorodimethyloctanedione (FOD), were employed to induce cellular injury and death. Lipid peroxidation was also measured as the formation of t...

Abstract Background Arginase is involved in the development of ischemia-reperfusion injury by regulating nitric oxide (NO) bioactivity via competition with NO synthase (NOS) for their common substrate L-arginine. Erythrocytes are known to contain high levels arginase that may reduce export cardioprotective bioactivity. Aim To determine role 1 erythrocytes cardiac protection against myocardial i...

PURPOSE To investigate the robustness of constrained and simultaneous intravoxel incoherent motion (IVIM) fitting methods and the estimated IVIM parameters (D, D* and f) for applications in brain and low-perfused tissues. MATERIALS AND METHODS Model data simulations relevant to brain and low-perfused tumor tissues were computed to assess the accuracy, relative bias, and reproducibility (CV%) ...

peaked positive chronotropic responses, i.e., rapidly induced initial acceleration followed by slowly induced but long-lasting one. The initial positive response to caffeine was hardly suppressd by either adrenergic beta-blocking agents, H 56/28 (alprenolol) and propranolol, or tetrodotoxin. On the contrary, the secondary positive chronotropic response was effectively inhibited by either adrene...

Placental transfer of lactate, glucose and 2-deoxyglucose was examined employing the in situ perfused placenta. Control and streptozotocin induced diabetic Wistar rats were infused with [U-14C]-glucose and [3H]-2-deoxyglucose (2DG). The fetal side of the placenta was perfused with a cell free medium and glucose uptake was calculated in the adjacent fetuses. Despite the 5-fold higher maternal pl...

Isolated perfused bovine uteri were used to study the irritation potential of antiseptics frequently used in veterinary practice for the treatment of endometritis. Comparable to the isolated perfused bovine udder (Kietzmann et al., 1993), the viability of uteri obtained directly after slaughtering of cows, was tested with the help of biochemical parameters. These parameters of the viability wer...