background: ferritin is an iron storage protein, which plays a key role in iron metabolism. measurement of ferritin level in serum is one of the most useful indicators of iron status and also a sensitive measurement of iron deficiency. monoclonal antibodies may be useful as a tool in various aspects of ferritin investigations. in this paper, the production of a murine monoclonal antibody (mab) ...

hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,echinococcus granulosus. this is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. in the current study, 20 sheep cystic livers or lungs were collected.hydatid fluid and protoscolices were isolated and the soluble protein was prepared. polypeptide ...

background: neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. methods: dot-elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect elisa and direct agglutination test (dat) were also evaluated by dot-elisa afterwards...

Diagnosis of scrub typhus is difficult because its symptoms are very similar to other acute febrile illnesses, such as leptospirosis, murine typhus, and other viral hemorrhagic fevers. To differentiate scrub typhus from other acute febrile diseases, a rapid and reliable serological diagnosis is important. We have developed a chimeric recombinant antigen cr56 and two other recombinant antigens, ...

fasciolosis is a disease caused by liver fluck of the genus of fasciola. diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. in this study, an in-house dot-elisa method; using excretion–secretory (es ag) and crude (cr ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. for this purpose, the sera...

PURPOSE Genetic alterations have been observed in a wide variety of neoplastic processes, including Burkitt's lymphoma, chronic myelogenous leukemia, promyelocytic leukemia, and solid tumors of the colon, skin, and breast. The polymerase chain reaction (PCR), dot blotting, and direct double-stranded DNA sequencing were used to assess ras gene activation in human uveal melanomas for three candid...