نتایج جستجو برای: dot blot assay

تعداد نتایج: 279986  

Journal: :Journal of Veterinary Diagnostic Investigation 1997

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Journal: :journal of cell and molecular research 0
maziar habibi-pirkoohi saeid malekzadeh-shafaroudi hasan marashi saeid zibaee afsaneh mohkami saba nejatizaxeh

an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...

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Journal: :Phytopathology® 1998

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ژورنال: مجله علمی دانشگاه علوم پزشکی همدان 2006
حق پناه, بهزاد, مساوات, بدرالسادات, عریضی, فرزاد , غیور, زهرا ,

Introduction & Objective : Hydatidosis, a disease caused by the cestod helminth echinococcus granulosus, is one of the most important parasitic zoonosis in man and a variety of animals. Sensitive and reliable serologic methods are necessary to confirm the diagnosis. In this study, Ag B and Psc Ag were purified as two specific parasitic antigens and evaluated by Dot blotting used on the serum of...

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Journal: :Cancer research 1992
R H Shoemaker A M Smythe L Wu M S Balaschak M R Boyd

A sensitive DNA dot-blot assay for repetitive human DNA sequences was developed and applied to the quantitative determination of spontaneous metastases of a human melanoma in various tissues of nude mice. The assay was useful for defining the time course and pattern of tissue distribution of metastatic cells as well as for assessing response to therapy. The methodology is relatively simple, can...

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Journal: :Nucleic Acids Research 2011

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Journal: :International Journal of Poultry Science 2006

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2018
Maria Beihaghi Hasan Marashi Abdolreza Bagheri Mojtaba Sankian

The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct of ccl21 was synthesized. This construct was cloned into pBI121 and...

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2011
Luciene C Scherer Rosa D Sperhacke Carla Jarczewski Patrícia I Cafrune Candice T Michelon Rubia Rupenthal Marta Osorio Ribeiro Antonio Ruffino Netto Maria LR Rossetti Afrânio L Kritski

BACKGROUND Direct smear examination with Ziehl-Neelsen (ZN) staining for the diagnosis of pulmonary tuberculosis (PTB) is cheap and easy to use, but its low sensitivity is a major drawback, particularly in HIV seropositive patients. As such, new tools for laboratory diagnosis are urgently needed to improve the case detection rate, especially in regions with a high prevalence of TB and HIV. OB...

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Journal: Archives of Razi Institute 2018
A. Rasouli, H. Hamidinejat, M. Ebrahimi, M. R. Tabandeh, M.H. Razi Jalali,

Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...

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  • " +i+ "
    • "; pgn_html+=pgn_li; } document.getElementById("pgn-ul").innerHTML=pgn_html var pgn_links = document.querySelectorAll('.mypgn'); pgn_links.forEach(function(pgn_link) { pgn_link.addEventListener('click', paginate) }) } function post_and_fetch(data,url) { showLoading() xhr = new XMLHttpRequest(); xhr.open('POST', url, true); xhr.setRequestHeader('Content-Type', 'application/json; charset=UTF-8'); xhr.onreadystatechange = function() { if (xhr.readyState === 4 && xhr.status === 200) { var resp = xhr.responseText; resp_json=JSON.parse(resp) resp_place = document.getElementById("search_result_div") resp_place.innerHTML = resp_json['results'] search_meta = resp_json['meta'] update_search_meta(search_meta) update_pagination() hideLoading() } }; xhr.send(JSON.stringify(data)); } function unfilter() { url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term var data={ "year":"unfilter", "term":term, "pgn":1 } filtered_res=post_and_fetch(data,url) } function deactivate_all_bars(){ var yrchart = document.querySelectorAll('.ct-bar'); yrchart.forEach(function(bar) { bar.dataset.active = false bar.style = "stroke:#71a3c5;" }) } year_chart.on("created", function() { var yrchart = document.querySelectorAll('.ct-bar'); yrchart.forEach(function(check) { check.addEventListener('click', checkIndex); }) }); function checkIndex(event) { var yrchart = document.querySelectorAll('.ct-bar'); var year_bar = event.target if (year_bar.dataset.active == "true") { unfilter_res = unfilter() year_bar.dataset.active = false year_bar.style = "stroke:#1d2b3699;" } else { deactivate_all_bars() year_bar.dataset.active = true year_bar.style = "stroke:#e56f6f;" filter_year = chart_data['labels'][Array.from(yrchart).indexOf(year_bar)] url=/search_year_filter/ var term=document.getElementById("search_meta_data").dataset.term var data={ "year":filter_year, "term":term, "pgn":1 } filtered_res=post_and_fetch(data,url) } } function showLoading() { document.getElementById("loading").style.display = "block"; setTimeout(hideLoading, 10000); // 10 seconds } function hideLoading() { document.getElementById("loading").style.display = "none"; } -->