نتایج جستجو برای: dot blot
تعداد نتایج: 72170 فیلتر نتایج به سال:
virulence of the opportunistic yeast, candida albieans, involves the interplay of many complex changes including the yeast-hyphae transition, which mainly involves protein changes. cell wall mannoproteins are found to be the main cause of adherence of c. albieans to epithel ial cells in the first step of an infection process. in the present study, cell wall mannoproteins of intact yeast were pu...
AIMS To evaluate different hybridisation techniques to detect and type human papillomavirus (HPV) DNAs amplified by consensus primer polymerase chain reaction (PCR) in biopsy and cytological specimens. METHODS A hybrid capture-immunoassay in microtitre wells was performed to detect HPV sequences amplified by PCR and typed by specific oligoprobes. Consensus primers were used to amplify a seque...
gp43 is the main diagnostic antigen for paracoccidioidomycosis (PCM). In vitro, gp43 expression in supernatant fluids of Paracoccidioides brasiliensis cultures can be unstable, and its regulation is poorly understood. We have been able to express soluble recombinant gp43 (gp43r) isoforms as N-mannosylated proteins secreted in the supernatants of Pichia pastoris cultures induced with methanol. T...
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