نتایج جستجو برای: dnmt1

تعداد نتایج: 1666  

Journal: :DNA research : an international journal for rapid publication of reports on genes and genomes 2005
Luciana R Vasques Raquel Stabellini Fei Xue X Cindy Tian Marina Soukoyan Lygia V Pereira

X chromosome inactivation (XCI) in human and mice involves XIST/Xist gene expression from the inactive X (Xi) and repression from the active X (Xa). Repression of the XIST/Xist gene on the Xa has been associated with methylation of its 5' region. In mice, Dnmt1 has been shown to be involved in the methylation and transcriptional repression of Xist on Xa. We examined maintenance of XIST gene rep...

Journal: :Blood 2006
Qian Zhang Hong Y Wang Anders Woetmann Puthiyaveettil N Raghunath Niels Odum Mariusz A Wasik

In this study, we demonstrated that STAT3, a well-characterized transcription factor expressed in continuously activated oncogenic form in the large spectrum of cancer types, induces in malignant T lymphocytes the expression of DNMT1, the key effector of epigenetic gene silencing. STAT3 binds in vitro to 2 STAT3 SIE/GAS-binding sites identified in promoter 1 and enhancer 1 of the DNMT1 gene. ST...

2014
Ercan Cacan Mourad W. Ali Nathaniel H. Boyd Shelley B. Hooks Susanna F. Greer

RGS10 is an important regulator of cell survival and chemoresistance in ovarian cancer. We recently showed that RGS10 transcript expression is suppressed during acquired chemoresistance in ovarian cancer. The suppression of RGS10 is due to DNA hypermethylation and histone deacetylation, two important mechanisms that contribute to silencing of tumor suppressor genes during cancer progression. He...

2015
ShunYu Zhao Jingjing Wu Fang Zheng Qing Tang LiJun Yang Liuning Li WanYin Wu Swei Sunny Hann

β-elemene, a compound derived from Rhizoma zedoariae, is a promising new plant-derived drug with broad-spectrum anticancer activity. However, the underlying mechanism by which this agent inhibits human lung cancer cell growth has not been well elucidated. In this study, we showed that β-elemene inhibits human non-small cell lung carcinoma (NSCLC) cell growth, and increased phosphorylation of ER...

Journal: :The Journal of biological chemistry 2003
Snezana Milutinovic Qianli Zhuang Alain Niveleau Moshe Szyf

The DNA methylation pattern is an important component of the epigenome that regulates and maintains gene expression programs. In this paper, we test the hypothesis that vertebrate cells possess mechanisms protecting them from epigenomic stress similar to DNA damage checkpoints. We show that knockdown of DNMT1 (DNA methyltransferase 1) by an antisense oligonucleotide triggers an intra-S-phase ar...

Journal: :Journal of biochemistry 2013
Naoyuki Hayashi Masahiko Kobayashi Awad Shamma Yoko Morimura Chiaki Takahashi Ken-ichi Yamamoto

NBS1 is the causative gene product of Nijmegen breakage syndrome (NBS), a recessive genetic disorder resulting in chromosomal instability and immunodeficiency. We isolated DNMT1 cDNA by two-hybrid screening by using NBS1 as bait to study its function in DNA replication and damage checkpoint. DNMT1 encodes DNA methyltransferase 1, which maintains the genomic methylation pattern and also regulate...

2008
Shujun Liu Zhongfa Liu Zhiliang Xie Jiuxia Pang Jianhua Yu Esther Lehmann Lenguyen Huynh Tamara Vukosavljevic Rebecca B. Klisovic Robert A. Baiocchi William Blum Pierluigi Porcu Ramiro Garzon John C. Byrd Danilo Perrotti Michael A. Caligiuri Kenneth K. Chan Lai-Chu Wu Guido Marcucci

Bortezomib reversibly inhibits 26S proteasomal degradation, interferes with NFB, and exhibits antitumor activity in human malignancies. Zinc finger protein Sp1 transactivates DNMT1 gene in mice and is functionally regulated through protein abundance, posttranslational modifications (ie, ubiquitination), or interaction with other transcription factors (ie, NFB). We hypothesize that inhibition of...

Journal: :Human molecular genetics 2008
Vincent Dion Yunfu Lin Leroy Hubert Robert A Waterland John H Wilson

Expanded CAG repeat tracts are the cause of at least a dozen neurodegenerative disorders. In humans, long CAG repeats tend to expand during transmissions from parent to offspring, leading to an earlier age of disease onset and more severe symptoms in subsequent generations. Here, we show that the maintenance DNA methyltransferase Dnmt1, which preserves the patterns of CpG methylation, plays a k...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2011
Kohei Takeshita Isao Suetake Eiki Yamashita Michihiro Suga Hirotaka Narita Atsushi Nakagawa Shoji Tajima

Methylation of cytosine in DNA plays a crucial role in development through inheritable gene silencing. The DNA methyltransferase Dnmt1 is responsible for the propagation of methylation patterns to the next generation via its preferential methylation of hemimethylated CpG sites in the genome; however, how Dnmt1 maintains methylation patterns is not fully understood. Here we report the crystal st...

2016
Rongfeng Xu Yuning Sun Zhongpu Chen Yuyu Yao Genshan Ma

Research has demonstrated that hypoxic preconditioning (HP) can enhance the survival and proliferation of cardiac progenitor cells (CPCs); however, the underlying mechanisms are not fully understood. Here, we report that HP of c-kit (+) CPCs inhibits p53 via the PI3K/Akt-DNMT1 pathway. First, CPCs were isolated from the hearts of C57BL/6 mice and further purified by magnetic-activated cell sort...

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