نتایج جستجو برای: dna primers

تعداد نتایج: 520069  

Journal: :iranian journal of allergy, asthma and immunology 0
hamid nawaz tipu department of immunology, armed forces institute of pathology, rawalpindi, pakistan muhammad mukarram bashir department of immunology, armed forces institute of pathology, rawalpindi, pakistan muhammad noma department of immunology, armed forces institute of pathology, rawalpindi, pakistan

serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...

2013
Asim A. Osman Imadeldin E. Aradaib Omer A. Musa

CONFLICT OF INTEREST none declared. INTRODUCTION This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. METHODS For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 4...

Journal: :Molecular medicine reports 2013
Rui Yang Jun-He Zhang Guo-Yan Yuan

The present study aimed to explore the factors that affect polymerase chain reaction using sequence-specific primers (PCR-SSP) and to establish an optimized PCR-SSP method for detecting multiple gene polymorphisms simultaneously. The amplification system parameters, including the concentrations of Mg2+, dNTPs, pfu Taq, primers and control primers, were optimized using the designed PCR-SSP react...

Journal: :The Journal of biological chemistry 1966
R Mantsavinos B Munson

A study has been made of the deoxyribonucleic acid primer requirements of partially purified deoxyribonucleic acid deoxynucleotidyltransferase fractionated from regenerating liver of the adult rat. Native, double stranded DNAs derived from either viral, bacterial, or mammalian sources serve more effectively as primers than either single stranded or heat-denatured DNAs for the incorporation of d...

2015
Yongzhen Xia Wenqiao Chu Qingsheng Qi Luying Xun

The QuikChange™ site-directed mutagenesis method is popular but imperfect. An improvement by using partially overlapping primers has been reported several times; however, it is incompatible with the proposed mechanism. The QuikChange™ method using complementary primers is proposed to linearly amplify a target plasmid with the products annealing to produce double-stranded DNA molecules with 5'-o...

2011
Jae-Yoon PARK Su-Jung HAM Ji-Yeon KIM Haryoung POO Chul-Joong KIM Moon-Hee SUNG

A previous study reported the cloning of a putative Prevotella nigrescens-specific DNA probe, Pn23, using random shotgun method. The present study evaluated the species-specificity of Pn23 for P. nigrescens using the clinical strains of Prevotella intermedia and P. nigrescens to develop Prevotella nigrescens-specific polymerase chain reaction (PCR) primers. The specificity was tested against 10...

Journal: :Acta biochimica Polonica 2006
Slawa Szostek Malgorzata Klimek Barbara Zawilinska Janusz Rys Jolanta Kope Ewa Daszkiewic

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R pri...

Journal: :BioTechniques 2009
Elena Hidalgo Ashrafi Natasha Paul

The polymerase chain reaction (PCR) is an indispensable DNA amplification technique that has been exploited in numerous areas, including molecular diagnostics. One major drawback of PCR is the competing amplification of undesired off-target products, which primarily occurs at ambient temperatures prior to PCR cycling (1). Hot Start PCR techniques aim to block the extension of primers in nonspec...

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