Magnetic nanoparticles separation technology is a method for quick and easy extraction biomolecules such as proteins, DNA and RNA. The present work describes total RNA isolation procedure from transformed rose petals in our laboratory using magnetic nanoparticles as a solid phase absorbant. Petals are the main sources of secondary metabolites, i.e. carotenoids, anthocyanins, flavonoids and phen...

DNA markers are being increasingly used in studies related to population genetics and conservation biology of endangered species. DNA isolation for such studies requires a source of biological material that is easy to collect, non-bulky and reliable. Further, the sampling strategies based on non-invasive procedures are desirable, especially for the endangered fish species. In view of above, a r...

A number of methods have been described for the isolation of genomic DNA from whole blood. However, these described methods are either unreliable or too expensive to be used routinely in small diagnostic laboratories of developing countries. We have evaluated the slightly modified salt-out method for DNA isolation so that it can be applied on whole blood samples using a three step procedure: (1...

Standardization of metagenomic DNA extraction protocol is a pre-requisite for a successful metagenomic study aiming to screen and exploit the variety of microorganisms inhabiting a particular soil environment. Six methods reported earlier were used for isolation of metagenomic DNA in the present study. These methods suffered with regard to either poor yield or quality of DNA. Therefore, we deve...

Many applications of gene analysis call for rapid and efficient methods for isolation of genome DNA from whole blood and complex biological samples. Relating to these demands, many protocols have been published (1-6) . However, either the methods involves toxic organic extractions and/or skillful manipulations such as spooling of DNA with an inoculation loop and the like to separate DNA from th...

Using a specific and sensitive polymerase chain reaction method, we detected reliably the presence of human cytomegalovirus (HCMV) DNA directly in serum samples collected at an early stage of HCMV infection, even before immunoglobulin M (IgM) antibodies were measurable. HCMV DNA was detected in serum from all patients with active HCMV infection; in 91% of these patients, HCMV DNA was found in t...

PCR based molecular markers are powerful tools for the analysis of genetic diversity for which isolation of good quality genomic DNA is essential. This paper presents a comparison of three DNA extraction methods for 1, 5 and 10 larvae of Aedes aegypti. First method employing DNAzol® involves simple and one step protocol for DNA isolation where maximum purity (A260/A280 1.9) was obtained with si...

Megabase-sized, complex, repetitive regions of genomes are poorly studied, due to the technical and computational challenges inherent to both assembling precise reference sequences and accurately assessing structural variation across contiguous megabase DNA regions. Here we describe a strategy to overcome these challenges, CISMR (CRISPR-mediated isolation of specific megabase-sized regions of t...

The paper analyzes how regional financial and trade integration have affected output fluctuations in East Asia. It also controls for possible effects of specialization patterns and exchange rate policies. The findings hint at a convergent direct effect of trade and FDI integration on output fluctuations. Real and financial integration also have sizable indirect effects on output fluctuations th...

The isolation of a single DNA molecule for cloning is technically difficult, and the subsequent screening of colonies for recombinant DNA clones is time consuming. Ion pair-reversed phase HPLC (IP-RP-HPLC) analysis of nucleic acids improves the resolution and isolation of PCR products to be cloned. We demonstrate that PCR products analyzed and collected using the IP-RP-HPLC approach (WAVE DNA F...