We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurot...

A dot blot hybridization test was developed for the detection of enterotoxigenic E. coli without the use of radioisotopes. Three biotin-labeled DNA (Bio-DNA) probes corresponding to structural genes specifying heat-labile and heat-stable enterotoxins of porcine and human origin were prepared by random priming; label incorporation was significantly higher than that obtained from the use of nick ...

Abstract Despite their high abundance and species richness, tilapiines have been compromised by various factors especially overfishing, climate change, uncontrolled fish transfers introductions. Fish introductions negatively impacted native tilapiine populations through competition, predation, hybridization, introgression compromising genetic integrity. The hybridization levels of different in ...