نتایج جستجو برای: dna aptamer

تعداد نتایج: 510626  

Journal: :Analytical chemistry 2014
Yueting Liu Jianping Lei Yin Huang Huangxian Ju

An "off-on" electrochemiluminescence (ECL) strategy was constructed for highly sensitive and selective detection of adenosine 5'-triphosphate (ATP) with a quantum dots (QDs) modified electrode and a DNAzyme signal probe. The immobilized QDs were functionalized with a DNA sequence (DNA1) and then aptamer for recognition of target analyte. The signal probe was prepared by assembling another DNA s...

2017
Marzieh Jafari Mohsen Rezaei Heibatullah Kalantari Maryam Tabarzad Bahram Daraei

Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and con...

2007
Milada Ikanovic Walter E. Rudzinski John G. Bruno Amity Allman Maria P. Carrillo Sulatha Dwarakanath Suneetha Bhahdigadi Poornima Rao Johnathan L. Kiel Carrie J. Andrews

A novel assay was developed for the detection of Bacillus thuringiensis (BT) spores. The assay is based on the fluorescence observed after binding an aptamer-quantum dot conjugate to BT spores. The in vitro selection and amplification technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment) was used in order to identify the DNA aptamer sequence specific for BT. The 60 ...

2012
Shoji Ohuchi Yusuke Mori Yoshikazu Nakamura

Aptamers are promising gene components that can be used for the construction of synthetic gene circuits. In this study, we isolated an RNA aptamer that specifically inhibits transcription of T7 RNA polymerase (RNAP). The 38-nucleotide aptamer, which was a shortened variant of an initial SELEX isolate, showed moderate inhibitory activity. By stepwise doped-SELEX, we isolated evolved variants wit...

Journal: :Current Opinion in Chemical Engineering 2014

2014
Mingli Yang Guohua Jiang Wenjing Li Kai Qiu Min Zhang Christopher M Carter Samer Z Al-Quran Ying Li

BACKGROUND The majority of patients with acute myelogenous leukemia (AML) still die of their disease. In order to improve survival rates in AML patients, new strategies are necessary to discover biomarkers for the detection and targeted therapy of AML. One of the advantages of the aptamer-based technology is the unique cell-based selection process, which allows us to efficiently select for cell...

2016
Elisa Biondi Joshua D. Lane Debasis Das Saurja Dasgupta Joseph A. Piccirilli Shuichi Hoshika Kevin M. Bradley Bryan A. Krantz Steven A. Benner

Reported here is a laboratory in vitro evolution (LIVE) experiment based on an artificially expanded genetic information system (AEGIS). This experiment delivers the first example of an AEGIS aptamer that binds to an isolated protein target, the first whose structural contact with its target has been outlined and the first to inhibit biologically important activities of its target, the protecti...

Journal: :The Analyst 2015
Zhaofeng Luo Hongmin Zhou Hao Jiang Huichao Ou Xin Li Liyun Zhang

Aptamers have attracted much attention due to their ability to bind to target molecules with high affinity and specificity. The development of an approach capable of efficiently generating aptamers through systematic evolution of ligands by exponential enrichment (SELEX) is particularly challenging. Herein, a fraction collection approach in capillary electrophoresis SELEX (FCE-SELEX) for the pa...

2017
Elena Zavyalova Nadezhda Samoylenkova Alexander Revishchin Askar Turashev Ilya Gordeychuk Andrey Golovin Alexey Kopylov Galina Pavlova

Anticoagulants are a vital class of drugs, which are applied for short-term surgical procedures, and for long-term treatments for thrombosis prevention in high risk groups. Several anticoagulant drugs are commercially available, but all have intrinsic disadvantages, e.g., bleeding risks, as well as specific ones, e.g., immune response to peptide/protein drugs. Therefore, the search for novel, e...

Journal: :Chemical communications 2010
Rui Ren Cuicui Leng Shusheng Zhang

The chronocoulometric detection of DNA was carried out based on circular strand-replacement DNA polymerization on a screen-printed electrode, and extended to quantify tumor cells with the aid of aptamer recognition.

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