The reaction mechanism for the enzymatic conversion of methyl phosphonate to phosphate and methane in Escherichia coli has eluded researchers over the last three decades despite significant genetic and in vivo studies. The phn operon governs the C-P lyase activity in E. coli. The essential genes within the phn operon are phnGHIJKLM. The proteins encoded by phnGHM were over-expressed in E. coli ...

Hydrogen-bond acidic polymers are important high affinity materials sensitive to organophosphates in the chemical warfare agent sensor detection process. Interactions between the sensor sensitive materials and chemical warfare agent simulants were studied by inverse gas chromatography. Hydrogen bonded acidic polymers, i.e., BSP3, were prepared for micro-packed columns to examine the interaction...

Soils that had a high binding capacity for inorganic orthophosphate (Pi) had reduced capacities to bind ionic alkyl phosphorus compounds. Only ionic methylphosphonate (MPn) and ionic phenylphosphonate exhibited moderate binding. Pseudomonas testosteroni used either MPn or Pi as a sole phosphorus source and exhibited diauxic utilization of MPn and Pi. The utilization of MPn was suppressed in the...

Electrolysis of Co(2+) in phosphate, methylphosphonate, and borate electrolytes effects the electrodeposition of an amorphous highly active water oxidation catalyst as a thin film on an inert anode. Electrodeposition of a catalytically competent species immediately follows oxidation of Co(2+) to Co(3+) in solution. Methylphosphonate and borate electrolytes support catalyst activity comparable t...

Multicasting can provide an efficient way of delivering data from a sender to a group of receivers. It has received much attention over the past decade because of an increasing demand for group communication applications such as multimedia streaming. However, native IP multicast has not become widespread largely due to its technical and operational issues. To overcome these obstacles of deploym...

Deoxyribooligonucleotides containing 19 repeating bases of A, T or U were prepared with normal phosphodiester (dA19, dT19, dU19) or methylphosphonate (dA*19, dT*19, dU*19) linkages. Complexes of these strands have been investigated at 1:1 and 1:2 molar ratios (purine:pyrimidine) by thermal melting and gel electrophoresis. There are dramatic sequence dependent differences in stabilities of compl...

Chemical warfare agents(CWA) such as nerve agents and vesicating show lethality by skin contamination. Skin protection, therefore, is one of the top priorities to deal with growing threat from CWA. In an attempt develop most effective topical protectant(TSP), candidate substances including PFPE(perfluorinated polyether), PTFE(polytetrafluoroethylene), glycerin, polysaccharides were evaluated in...