The present study including two experiments was designed to determine the effect of media containing different rare earth elements (REE) on proliferation and fatty acids accumulation in 3T3-L1 cell cultures. In Experiment 1, 3T3-L1 preadipocytes in 96-well plates (1.5×10 cells/ml) were cultured with Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) for 24 h. Then...

To study enterotoxigenic Escherichia coli (ETEC) association to the gut of pigs, a simple and reproducible experimental model would be helpful. The aim of this experiment was to establish a model for studying the association of ETEC to the gut epithelium of pigs. Intestinal segments were prepared from 4 weaned pigs, which were tested susceptible to E. coli O149:F4 (homo- and heterozygotic; 2 pi...

Submit Manuscript | http://medcraveonline.com Abbreviations: CMGG: Carboxymethyl Guar Gum; BMMscs: Bone Marrow Derived Mesenchymal Stem Cells; DMEM: Dulbecco’s Modified Eagle Medium; FBS: Foetal Bovine Serum; Pen-Strep: Penicillin-Streptomycin; LPS: Lipopolysaccharide; Cocl2.6H2O: Cobalt Chloride Hexahydrate; PBS: Phosphate Buffered Saline; EDTA: Ethylene Diamine Tetra Acetic Acid; MTT: 3-(4, 5...

The structure of the lower part hair follicle undergoes cyclic formation and decay. During anagen, is surrounded by capillary blood vessels that supply oxygen nutrients. However, disappear during catagen, concentration in expected to decrease significantly. There little information on response human DPCs hypoxic condition follicle. In this research, we evaluated its influence expression TGF-β2 ...

Background Infections with Onchocerca volvulus nematodes remain a threat in Sub-Saharan Africa after three decades of ivermectin mass drug administration. Despite this effort, there is still an urgent need for understanding the parasite biology especially mating behaviour and nodule formation as well development more potent drugs that can clear developmental (L3, L4, L5) adult stages inhibit re...

Mesenchymal stem cells (MSCs) secrete a variety of cytokines and growth factors in addition to self-renewal and multiple forms of differentiation. Some of these secreted bioactive factors could improve meiotic maturation in vitro and subsequent embryo developmental potential. The aim of the present study was to determine whether in vitro maturation (IVM) of mouse oocyte with or without cumulus ...

We tested the effects of mouse embryonic stem cells (mES) grafts in mice spinal cord injury (SCI). Young adult female C57/Bl6 mice were subjected to laminectomy at T9 and 1-minute compression of the spinal cord with a vascular clip. Four groups were analyzed: laminectomy (Sham), injured (SCI), vehicle (DMEM), and mES-treated (EST). mES pre-differentiated with retinoic acid were injected (8 x 10...