OBJECTIVE To determine prevalence of Dirofilaria immitis infection among shelter cats. DESIGN Cross-sectional study. ANIMALS 239 cats euthanatized at an animal shelter in southeastern Michigan. PROCEDURE A gross necropsy focusing on the thoracic cavity, heart, lungs, and pulmonary vessels was performed within 5 hours after cats were euthanatized. Blood was collected directly from the hear...

The amino acid analysis of the purified allergen from Dirofilaria immitis revealed that it had a total number of 98 amino acid residues. The allergen was remarkably rich on glutamic acid (15 residues), lysine (14 residues) and aspartic acid (12 residues). No half-cystine was obtained. The ratio of the acidic residues to the basic ones was 1.4. The weak positive charge of the allergen is contrib...

Dirofilaria immitis was diagnosed in 4 of 13 indigenous dogs from the Province of Zambézia, Mozambique, by acid phosphatase staining of microfilariae. The finding reconfirms the occurrence of the parasite in Mozambique after 3 decades and emphasises the need for extensive surveys. Additionally, in 1 of the infected dogs, microfilariae of Dipetalonema reconditum were detected, which is the 1st r...

We have cloned and characterized the gene for an immunodominant antigen of O. volvulus that is recognized by the sera of 96% of patients with onchocerciasis. Its 1.2-kb mRNA constitutes 0.3% of adult worm poly(A)+ RNA and its cDNA sequence reveals that it is not a highly conserved structural protein such as actin or tubulin. Similar but not identical genes occur in the genomes of related filari...

A total of 38 species mosquitoes the Culicidae family two subfamilies Anophelinae and Culicinae belonging to 6 genera, were identified on territory Kharkiv Region from 2009 2019. Dirofilaria larvae found in 932 insects, which was 4.46 % surveyed mosquitoes. The prevalence infection female by dirofilaria ± 0.24 %, while mean intensity dirofilariae 5.4 2.1. Infection with is currently detected 11...

The vector competence of Culex quinquefasciatus from five localities in Brazil to Dirofilaria immitis was evaluated experimentally. Females from each locality were fed on an infected dog ( approximately 6 microfilariae/microl blood). A sample of blood fed mosquitoes were dissected approximately 1 h after blood meal. These results demonstrated that all had ingested microfilariae (mean, 4.8 to 24...

It has been shown recently that 1-diethylcarbamyl-4-methylpiperazine hydrochloride produces marked effects against Litomosoides carinii in cotton rats (Hewitt, White, Wallace, Stewart, Kushner, and SubbaRow, 1947) and (Hewitt, Kushner, Stewart, White, Wallace, and SubbaRow, 1947), Dirofilaria immitis in dogs (Hewitt, Kushner, Stewart, White, Wallace, and SubbaRow, 1947), adult Trichinella spira...

Dirofilaria immitis is an important filarial nematode in dogs. In this study, age and sex distribution of this zoonotic nematode among dogs were investigated in northwest of Iran in Meshkin-Shahr city. Molecular characteristics of the isolates, based on cytochrome oxidase subunit 1 (COX1) gene were compared to the isolates from other areas of the world.Blood samples were collected from 91 dogs ...